[Duodenal duplication in adults. Laparoscopic treatment].

The authors report a case of duodenal duplication in a adult. This lesion is rare. The clinical course is dominated by a risk of cancer.

Importance of hydropathic complementarity for the binding of the neuropeptide substance P to a monoclonal antibody: equilibrium and kinetic studies.

In a previous study (Boquet et al., 1995, Molec. Immunol.

Comparison of different immunoenzymatic methods for the determination of the fine specificity and affinity constants of polyclonal antibodies against pseudopeptide haptens.

We evaluated two phosphinopeptides and one phosphonopeptide, which are transition state analogs of a proteolytic reaction, for their ability to generate murine polyclonal antibodies. The specificity of these antisera was determined by indirect and competitive ELISA. Cross-reactivity analysis by these ELISA showed that the antisera recognized selectively haptens containing a phosphate group.

Development and standardization of an immuno-quantified solid phase assay for HIV-1 aspartyl protease activity and its application to the evaluation of inhibitors.

The catELISA technique was modified and standardized for measuring HIV-1 aspartyl protease activity and evaluating the potency of synthetic peptide inhibitors. This immuno-quantified solid phase assay combines the use of an immobilized C-terminal biotinylated peptide as substrate, a crude enzyme preparation, and a highly specific antiserum elicited against the C-terminal product of the enzyme reaction. A standard curve of this C-terminal product was constructed to determine the enzyme activity.

Characterization of a monoclonal antibody produced in an attempt to mimic the active site of HIV aspartyl protease using haptens based on inhibitor models.

The high binding affinity and specificity of antibodies for a great variety of ligands has been widely exploited in structure-activity relationship studies of biomolecules and more recently in the development of new catalysts for several chemical reactions. It is assumed that antibodies generated against haptenic protease inhibitors would recognize both these haptens and the substrate of the model proteolytic reaction. We have produced antibodies against HIV PRp12 aspartyl protease substrate analogues, chemically modified at the scissile bond, Phe-Pro.