Comparison of Gene Expression Profiles of Uropathogenic CFT073 after Prolonged Exposure to Subinhibitory Concentrations of Different Biocides.

Biocides are chemical compounds widely used for sterilization and disinfection. The aim of this study was to examine whether exposure to subinhibitory biocide concentrations influenced transcriptional expression of genes that could improve a pathogen's drug resistance or fitness. We used DNA microarrays to investigate the transcriptome of the uropathogenic strain CFT073 in response to prolonged exposure to subinhibitory concentrations of four biocides: benzalkonium chloride, chlorhexidine, hydrogen peroxide and triclosan.

Investigation of the quality of stored red blood cells after simulated air drop in the maritime environment.

Background: Maritime medical capability may be compromised by blood resupply. Air-dropped red blood cells (RBCs) is a possible mitigation factor. This study set out to evaluate RBC storage variables after a simulated parachute air drop into the sea, as limited data exist.

Low concentrations of citrate reduce complement and granulocyte activation in vitro in human blood.

Background: The use of acetate in haemodialysis fluids may induce negative effects in patients including nausea and increased inflammation. Therefore, haemodialysis fluids where acetate is substituted with citrate have recently been developed. In this study, we investigated the biocompatibility of citrate employing concentrations used in haemodialysis.

Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition.

Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli responsible for >80% of all cases. One extreme of UTI is asymptomatic bacteriuria (ABU), which occurs as an asymptomatic carrier state that resembles commensalism. To understand the evolution and molecular mechanisms that underpin ABU, the genome of the ABU E.

The 30 minute rule for red blood cells: in vitro quality assessment after repeated exposure to 30°C.

Background: Red blood cells (RBC) may be out of temperature control only for 30 minutes before they must be discarded, but evidence for this rule is weak. We investigated the effect on RBC quality of multiple exposures to 30°C.

Study Design And Methods: RBC units made after 24 hours of whole blood ambient hold were pooled and split into adult and pediatric units and exposed to permitted deviations (5-hr core temperature 10°C, 12-hr surface temperature 10°C).