O-substituted Tertiary Amine-chitosans as Promising Nanocarriers for siRNA Delivery.

Introduction: The clinical translation of chitosan-based formulations for siRNA delivery has been partially limited by their poor stability/solubility at physiological conditions, although they have good biocompatibility and cost-effectiveness.

Method: In this study, the chitosan was O-substituted with diisopropylethylamine (DIPEA) groups, which improved its solubility at pH 7.4 by increasing the degree of ionization and enhanced the ability of chitosan to load siRNA at very low amine/phosphate (N/P) ratios.

Integrative analysis of miRNAs and proteins in plasma extracellular vesicles of patients with familial hypercholesterolemia.

Background And Aims: Familial Hypercholesterolemia (FH) is a monogenic disease that leads to early-onset atherosclerosis. Causative mutations in FH-related genes are found in 60-80 % of patients, while epigenetic factors may contribute to mutation-negative cases. This study analyzed miRNAs and proteins from plasma-derived extracellular vesicles (EVs) of FH patients to explore their contribution in FH diagnosis.

Early warning system using primary health care data in the post-COVID-19 pandemic era: Brazil nationwide case-study.

Syndromic surveillance using primary health care (PHC) data is a valuable tool for early outbreak detection, as demonstrated by the potential to identify COVID-19 outbreaks. However, the potential of such an early warning system in the post-COVID-19 era remains largely unexplored. We analyzed PHC encounter counter of respiratory complaints registered in the database of the Brazilian Unified National Health System from October 2022 to July 2023.

Brazilian version of the Kerlan-Jobe orthopedic clinic shoulder and elbow score: translation and cross-cultural adaptation.

Background: The Kerlan-Jobe Orthopedic Clinic (KJOC) Shoulder and Elbow Score is commonly used to assess the functional status of athletes with conditions affecting the shoulder and elbow. However, a Brazilian Portuguese version of the KJOC questionnaire is currently unavailable.

Objectives: This study aimed to develop a Brazilian Portuguese version of the KJOC questionnaire.

Child Well-Being, Family Functioning, and Contextual Strain: A Study of Multi-Assisted Low-Income Families.

Objectives: This study aimed to examine the effects of poverty on child well-being and family functioning among low-income families. Specifically, it explored the role of parental stress, family dynamics, and contextual strain on children's behavioral and emotional outcomes. Using a sample of families receiving welfare support, the research sought to assess the impact of conditional cash transfer programs and the involvement of Child Protective Services on child development and family well-being.