Publications by V G Sunnichan

Publications by authors named "V G Sunnichan"

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A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.): embryogenic calli as a source to generate large numbers of transgenic plants.

S Leelavathi V G Sunnichan R Kumria G P Vijaykanth R K Bhatnagar

Plant Cell Rep

February 2004

Article Synopsis

A new protocol for transforming and regenerating cotton has been developed, involving embryogenic calli co-cultivated with Agrobacterium carrying the cry1Ia5 gene.
After 3-6 weeks of culture under stress and antibiotic selection, around 75 globular embryo clusters were produced, leading to an average of 12 plants per Petri plate.
The method is efficient, easy to execute, and allows for multiple transformations, making it suitable for various cotton varieties.

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High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress.

R Kumria V G Sunnichan D K Das S K Gupta V S Reddy

Plant Cell Rep

March 2003

A highly efficient somatic embryo production and maturation procedure has been developed to regenerate plantlets from cotton ( Gossypium hirsutum). This procedure involves the acceleration of differentiation through manipulations of nutrient and microenvironment conditions. Embryogenic calli, initiated from hypocotyls or cotyledonary leaf sections on MS medium containing 0.

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Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis.

V G Sunnichan K R Shivanna H Y Mohan Ram

Plant Cell Rep

September 1998

Nodal explants from selected trees of gum karaya (Sterculia urens Roxb.) in the adult growth phase cultured on Murashige and Skoog (MS) medium supplemented with 6.62 μM N-benzylaminopurine (BAP) produced an average of six adventitious shoots in 30 days.

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