The mechanism of hemolysis of erythrocytes by sodium dodecyl sulfate.

The results of a study of vesicular-hemolytic action of anionic detergent sodium dodecyl sulfate on human erythrocytes are presented. Possible molecular events underlying detergent-induced pore formation in the erythrocyte membrane are discussed.

Dependence of erythrocyte vesiculation and hemolysis parameters on the concentration of sodium dodecyl sulfate. Vesicular-competitive hemolysis.

The kinetic and concentration dependences of erythrocyte vesiculation and hemolysis induced by sodium dodecyl sulfate were studied. The similarity of the slopes of the dose dependence of the SDS-induced vesiculation and slow hemolysis rates in the double logarithmic coordinates suggested a close relation between the processes of vesiculation and pore formation for slow hemolysis by the detergent. Further evidence of the competitive nature of the detergent-induced vesiculation and fast hemolysis by sodium dodecyl sulfate was obtained.

PH-dependence of detergent-induced hemolysis and vesiculation of erythrocytes.

The influence of pH of the medium on the parameters of detergent-induced fast hemolysis and vesiculation of human erythrocytes was studied. In the range of pH 6.3-7.

[Effect of erythrocyte membrane structure on the dose dependence of photohemolysis].

The influence of different factors modifying erythrocyte membrane structure on the value of power index of doze dependence of photohemolysis of hematoporphyrin-sensitized erythrocytes has been investigated. It has been established that thermoinduced spectrin aggregation results in increase the index, whereas vesiculation under metabolic exhaustion of erythrocytes greatly decreases value n. A more considerable decrease of the parameter was observed when photohemolysis of erythrocytes was occurred in alkaline medium.