Control of Allergen-Induced Histamine Secretion by Human Basophils with F(ab) of Anti-IgE Antibodies.

A strategy was developed to obtain univalent F(ab) fragments against IgE, which would interfere with IgE-mediated activation of target cells, but lacked their activity due to cross-linking of IgE bound to Fc(epsilon)-receptors. Two kinds of mouse anti-human IgE monoclonal antibodies (mAb) against CH(2) or CH(3) domains induced histamine release from basophils of allergic patients and healthy donors. F(ab')(2) fragments of the mAb (0.

[A shock reaction from insect bites in patients with urticaria pigmentosa].

The examination of 11 urticaria pigmentosa (UP) patients included allergological history, skin prick, scarification tests and intracutaneous tests with noninfectious and bee poison allergens, total and specific serum IgE measurements, in vitro reaction of histamine release from peripheral blood basophils induced by bee poison. The response of mastocytosis patients to insect sting was characterized by a rapid (within 5 min) development of severe systemic reactions or shock. The skin reactions and serum antibodies to bee poison were not registered in 9 of 11 patients.

Histamine releasing activity of blood mononuclear cells is acquired by means of activation of mast cells.

It has been shown that peripheral blood mononuclear cells (PBMC) obtained from atopic patients with acute clinical manifestations of pollinosis, atopic dermatitis or bronchial asthma, and preincubated in vitro for 18 hours, acquired the ability to induce histamine release from auto-basophils and basophils of healthy donors. Both PBMC and their supernatants possessed this histamine releasing activity (HRA). During remission, HRA could be reproduced in sensitive patients after positive cutaneous tests with a specific allergen.

[A comparative study of the efficacy of Tilade and intal in atopic bronchial asthma].

The authors compared the responses of patients with atopic asthma to two cromolyn drugs of different generations, tilade and intal. As shown by clinical, allergological and immunological findings, tilade (sodium nedocromil) compared to intal is more active against atopic bronchial asthma complicated by obstructive bronchitis. It can more efficiently reduce specific and nonspecific bronchial hyperreactivity, is more potent against inflammation.