Inhibitory effect of tunicamycin on the adhesivity and aggregation of embryonic brain cells.

Tunicamycin (TM) - (0.1, 1.0 and 10 micrograms/ml) inhibits insignificantly the adhesivity of embryonic mice brain cells during the first 120 min of incubation.

Enzyme activity pattern in developing mouse brain in situ in embryonic brain aggregated cells at 37 degrees C and 0 degree C.

In aggregates of nervous tissue, cultivated for 1--7 days at 0 degree C and 37 degrees C, respectively, the activities of seven enzymes of energy liberating metabolism were estimated, in order to evaluate their metabolic "profiles" and changes during cultivation. The enzymes used as markers of different pathways of energy liberation from substrates were: lactate dehydrogenase - LDH - (EC 1.1.

Ultrastructural and protein synthetic changes in embryonic brain: cells aggregated at 0 degree C.

Cells dissociated from brains of 16 to 18 day-old mice embrya were rotated at 37 degrees C and 0 degree C for 7--8 days. While cells aggregated at 37 degrees C formed compact aggregates, cells aggregated at 0 degree C were found in clusters or were randomly distributed. Cells aggregated in the cold did not differ markedly from the controls in their ultrastructural organisation till the 2--3 day in vitro (DIV).

Reaggregation of human, chick, and human embryonic brain cells. Factors influencing the formation of a histiotypic unit.

1. Aggregation of embryo human, mouse, and chick brain cells was studied. The optimum age interval of donors from different species was determined.