[Changes in polarization of myometrial cells plasma and internal mitochondrial membranes under calixarenes action as inhibitors of plasma membrane Na+, K+-ATPase].

The influence of supramolecular macrocyclic compounds--calix[4]arenes C-97, C-99, C-107, which are ouabainomymetic high affinity inhibitors of Na+, K(+)-ATPase, on the polarization level of plasmic and mitochondrial membranes of rat uterine smooth muscle cells was investigated. The influence of these compounds on the myocytes characteristic size was studied. By using a confocal microscopy and specific for mitochondrial MitoTracker Orange CM-H2TMRos dye it was proved that the potential-sensitive fluorescent probe DiOC6(3) interacts with mitochondria.

[Registration of K(+)-equilibrium potential in myometrium cell plasma membrane and study of its modulation of NO(x) and H2O2, using flow cytometry method].

Prospects of the use of flow cytometry analysis for investigation of forming K(+)- equilibrium membrane potential on the experimental model of myometrium plasma membrane vesicles in the presence of valinomycine using potential-sensitive probe of DiOC6(3). Transmembrane potential magnitude corresponds to magnitude by Nernst's equation. H2O2 and NO2-, probably, increase permeability of membrane for K+ and result in potential dissipation.

[Fluorescent derivatives of diphtheria toxin subunit B and their interaction with Vero cells].

Diphtheria toxin's B subunit provides toxin interaction with its receptor on the cell surface and translocation of toxin's A subunit from endosome to cytozole of sensitive cells. Functional analogues of B subunit with fluorescent label are considered as perspective tools for studying the above mentioned processes. The aim of the work was to obtain fluorescent B subunit analogues and to detect the specificity of their interaction with Vero line cells.

[Identification of mechanism of enzyme/transport process in the system of "enzyme/ transporter--substrate--effector" using the "ratio" method].

Using equilibrium assumption the analysis of kinetics of enzyme/transport process has been developed. In the course of this process the enzyme can interact simultaneously with substrate S and effector E and as a result two products are generated: P1 (from substrate) and P2 (from effector) after catalytic effect of enzyme or transportation across the biological membrane. It has been demonstrated that the ratio (R = V0,1/V0,2) of initial rates of formation of reaction products P, (V0,1) and P2 (V0,2) represented in linearized form as a dependence on concentration of both substrate S0 and effector E0 allows to identify a specific mechanism of the present process.

[Studies of Ca2+ -dependent smooth muscle mitochondria swelling using flow cytometry and spermine effects on this process].

In the experiments, which were carried out using flow cytometry on isolated uterus mitochondria of nonpregnant rats, conditions for studying Ca2+-induced increase in nonspecific permeability of mitochondial membrane were tested. Fluorescent probe nonyl acridin orange was used to determine the purity of isolated mitochondria. Mitochondrial swelling induced by addition of Ca2+ or alamethicin was detected as a decrease in light side scattering (SS).