[Xanthine oxidase as an indicator of neonatal adaptation].

The reactions reflecting adaptation may assume pathological traits under certain conditions. It is necessary to have reliable predictors that characterize adaptive processes. As this criterion, it is expedient to use the most important marker of free radical oxidation, such as xanthine oxidase (XO) activity.

[Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus].

A combinatorial immune library of human single-chain antibody fragments (scFv) was constructed on the base of genes encoding variable domains of heavy and light chains of immunoglobulins cloned from the lymphocytes of four vaccinia virus (VACV) vaccinated donors. The size of the library was 3 x 10(7) independent clones. After the library was enriched with the clones producing scFv against recombinant analogue of variola virus surface protein prA30L, a panel of unique antibodies specific to both prA30L and VACV was selected from the library.

[Construction and characterization of combinatorial naive phage library of human scFvs].

A combinatorial phage display library of human single-chain antibody fragments (scFv) was constructed on the basis of variable domains of heavy (Vh) and light (VI) genes cloned from the lymphocytes of six healthy donors. The size of the library was 2? 10(8) independent clones. Single-chain antibodies against recombinant human TNF?, vaccinia virus and virus-like particles formed by core protein of hepatitis B virus were selected from the library.

[Comparative study of the changes in structural parameters of collagen fibers during natural aging and pneumosclerosis formation].

Changes in diameter of the collagen fibers and their number in fascicles of the rat lung in the process of natural ageing and formation of radiation-induced pneumo-sclerosis were studied electron microscopically. The decrease of the fibril thickness and the increase of their number in fascicles progressed during the postirradiation period and reached the values absent in the control animals.