THE INCIDENCE OF PRIMARY AND SECONDARY RESISTANCE TO THE ANTI-CYTOKINE THERAPY IN PATIENTS WITH INFLAMMATORY BOWEL DISEASE.

Long-term experience of using irifliximab in patients with inflammatory bowel disease (IBD) have shown that 20-30% of patients couldn't achieve clinical improvement (primary failure) or have developed acquired drug resistance (secondary failure). The frequency of antibody formation to infliximab is to 28-61%, for adalimumab 4-12%, to certolizumab the guests to 12.3%, for etanercept to 2.

[Significance of a method for determination of deamidated gliadin peptide in the diagnosis of celiac disease].

Aim: To define the value of a new enzyme immunoassay in determining the level of anti-deamidated gliadin peptide (DGP) antibodies (Abs) in the diagnosis of celiac disease.

Subjects And Methods: One hundred and twenty-four patients treated at the Department of Intestinal Pathology, Central Research Institute of Gastroenterology, were examined. Enzyme-linked immunosorbent assay (ELISA) was employed to determine Abs to tissue transglutaminase (tTG) and DGP of the IgA and IgG classes in the sera of all the patients.

[Value of adhesion molecules for evaluating the efficiency of therapy for ulcerative colitis and Crohn's disease].

Aim: To define the value of adhesion molecules (sVCAM-1 integrin, P-selectin, E-selectin, and L-selectin) for the prediction and evaluation of the efficiency of treatment in patients with ulcerative colitis (UC) and Crohn's disease.

Subjects And Methods: Twenty-six patients with UC and 14 patients with CD were examined. Of them, 16 patients took infliximab (INF) in a dose of 5 mg/kg of body weight according to the standard scheme; 14 patients received cultured mesenchymal stem stromal cells (MSSCs) in a quantity of 150 x 10(8) cells, and 10 had azathioprine (AZA) 2 mg/kg and glucocorticosteroids (GCS) 1 mg/kg of body weight.

[Peculiarities of the clinical course of ulcerative colitis, depending on the spectrum of cross-linked autoantibodies from the same family to antigens of neutrophils].

There were found that patients with ulcerative colitis (UC) in the presence of two types of autoantibodies to antigens of neutrophils is observed in less pronounced activity in the course of the disease compared with those patients with a spectrum of autoantibodies one of the wider family. The patients with three or more types of antibodies from the same family, cross-linked, ware accompanied by an increase in clinical and endoscopic disease activity. It is shown that a--greater range of autoantibodies one family determines not only the degree of activity of the YAK, but the nature and intensity of its extraintestinal manifestations.

[Clinical immunological diagnostics of overlap syndrome during autoimmune hepatic disorders].

The complex determination of serum autoantibodies to hepatic antigens using enzyme immunoassay and immunoblot allows to increase the frequency of overlap syndrome identification during autoimmune hepatic disorders and its early diagnostics, that has a big clinical, diagnostic and prognostic importance. The levels of overlap autoantibodies combine with biochemical index and with disease activity and intensity of autoimmune processes during overlap syndrome of primary biliary cirrhosis/autoimmune hepatitis (PBC/AIH).