Influence of growth factors erythropoietin and granulocyte macrophage colony stimulating factor on mechanical strength and healing of colonic anastomoses in rats.

Objective: To find out what influence erythropoietin and granulocyte macrophage colony stimulating factor (GM-CSF) had on the healing of left colonic anastomoses in rats.

Design: Experimental study.

Setting: University hospital of Ioannina, Greece.

Immunohistochemical detection of ras P21 oncoprotein in human skin lesions.

This study was undertaken to investigate the role of the human ras family gene product [P21] in various benign, precancerous and malignant skin lesions. A streptavidin-biotin peroxidase method was performed using the monoclonal antibody (Mab)Y13259 in paraffin tissue sections of a total of 69 skin lesions (5 benign hyperplasias, 12 seborrheic keratoses, 9 solar keratoses, 20 basal cell carcinomas and 23 squamous cell carcinomas). The adjacent normal skin was also studied in all cases.