[GIS: CAPABILITIES OF DATA ANALYSIS OF PHENO- AND GENOTYPING Of EL TOR 01 SEROGROUP CHOLERA VIBRIOS ISOLATED FROM AQUATIC OBJECTS OF THE ENVIRONMENT IN RUSSIA FEDERATION].

Aim: Application of the authors' GIS <> for systematization of atoxigenic strains ofserogroup 01 choleravibrios (ctxAB-tcpA-, ctxAB-tcpA+), isolated from aquatic objects of the environment by pheno- and genotype.

Materials And Methods: A sample of 304 Vibrio cholerae 01 strains was studied. Isolation of 39 genes related to pathogenicity was carried out Discrimination ability of a set of genes was determined by Simpson formula.

[TYPING OF VIBRIO CHOLERAE NON O1/NON O139 STRAINS, ISOLATED IN ROSTOV REGION IN 2014].

Aim: Comparative study of antibiotics resistance and VNTR-typing of Vibrio cholerae non O1/ non O139 strains, isolated on the territory of Rostov region in 2014.

Materials And Methods: Antibioticogramms of strains were determined by serial dilution method in dense nutrient medium according to MG 4.2.

[DETERMINATION OF TYPES OF EPIDEMIC MANIFESTATIONS OF CHOLERA IN REGIONS OF THE CRIMEA FEDERAL DISTRICT (REPUBLIC OF CRIMEA)].

The aim of the study was determination of the type of epidemic manifestations of cholera in the Republic of Crimea based on evaluation of epidemic manifestations of cholera risk of introduction and spread of the infection. It was concluded, that, based on the cholera outbreaks, that had taken place, contamination of surface water bodies (fresh and sea) and sewage by Vibrio cholerae O1 ctxA+ and Vibrio cholerae O1 ctXA- potential epidemic danger of introduction of the infection by various types of international transport, population migration, the presence of epidemiologic risk in realization of water pathway of transmission of cholera causative agent and several other social conditions, the Republic of Crimea remains in the group of territories of type I by epidemic manifestations of cholera.

[The immune-enzyme techniques of analysis in diagnostic of cholera.].

The optimal conditions of arrangement of direct alternative of flatbed enzyme-linked immunosorbent assay and dot-immunoanalysis with application of monoclonal peroxidase conjugates for express identification of comma bacillus of serogroups O1 and O139 both in hospital and field conditions without device support. The direct technique enzyme-linked immunosorbent assay in flatbed alternative shortens time of analysis up to 70-80 minutes and in case of dot enzyme-linked immunosorbent assay on membrane - up to 70-90 minutes. It is established that in case of analysis in conditions of room temperature (20-25 C) sensitivity of techniques remains at initial level.