A Comprehensive Study of Meningioma Biomineralization: Morphological, Crystallographic, and Immunohistochemical Aspects.

Biomineralization of brain tissues occurs both in normal and pathological conditions. Dura mater biomineralization is widespread and occurs in 1-72% of cases, depending on the patient's age and research method. The amount of biomineral deposits under the conditions of tumor growth in the meninges only increases, reaching 100% in the case of psammomatous meningiomas.

A Simple Method to Determine the Fractions of Labile and Mineral-Bound Microelements in Bone Tissue by Atomic Absorption Spectrometry.

In this work a simple and inexpensive method to assess the concentration ratio of the labile and mineral-bound microelements of the bone tissue was developed. The approach is based on the separation of the components of bone tissue by their selective solubility with the subsequent determination of microelements with atomic absorption spectrometry. The total concentrations of Mg, Zn, Fe, Sr, Al, Cu, and Mn and the concentrations of these elements in aqueous solutions with pH 6.

[Mass spectrometry in the study of bioclasters of amino acids and peptides].

The study of homo- and heterocluster quasimolecular ions of 20 L-amino acids (A) and five dipeptides by the TOF-PDMS method indicated that the intensity of quasimolecular ions of the corresponding homo-([An + H]+ and [Bm + H]+, where A and B are biomolecules (A, dipeptides), n and m = 1 ...

[An improved method of preparing protein and peptide probes in mass spectrometry with ionization of division fragments by californium-252 (TOF-PDMS)].

The addition of organic acids (picric, oxalic, citric, or tartaric) to peptide and protein samples was found to significantly increase the yield of their quasi-molecular ions (QMI) in time-of-flight 252Cf plasma desorption mass spectrometry. The yield of the ions depended on the pKa of the acid added.

Peptide sequencing by partial acid hydrolysis and high resolution plasma desorption mass spectrometry.

A method of deriving peptide sequence information using partial acid hydrolysis in combination with accurate mass measurements and immonium ion analysis provided by high-resolution plasma desorption mass spectrometry has been developed. The technique is very simple in terms of the chemistry and involves a short-time (3-30 min) incubation of the peptide in 1N-6N HCl at 100-110 degrees C with subsequent mass spectrometric analysis. Partial acid hydrolysis is found to produce sequence-specific segments, often ladder-like, although not always a complete set.