Gastrulating Rat Embryo in a Serum-free Culture Model: Changes of Development Caused by Teratogen 5-Azacytidine.

Developmental processes in gastrulating rat embryos were investigated by using an original, serum-free, chemically defined model system. 9.5-day-old rat embryos, without extraembryonic membranes, were cultivated at the air-liquid interface in a serum-free medium, with and without a protein supplement, for 2 weeks.