Inorganic-organic core/shell nanoparticles: progress and applications.

In recent decades a great deal of research has been dedicated to the development of core-shell nanoparticles (NPs). We decided to focus our attention on NPs with inorganic cores and organic shells and divide them by area of application such as electrical applications, drug delivery, biomedical applications, imaging, chemistry and catalysis. Organic shells, consisting in most cases of polymers (natural or synthetic), proteins or complex sugars, can improve the performance of inorganic NPs by enhancing their biocompatibility, acting as anchor sites for molecular linkages or protecting them from oxidation.

Relationship between Vac A toxin and ammonia in Helicobacter pylori-induced apoptosis in human gastric epithelial cells.

VacA toxin is one of the most important virulence factors produced by H. pylori even though neither its role nor its action mechanisms are completely understood. First considered as a toxin inducing only cell vacuolation, VacA causes apoptosis of gastric epithelial cells by targeting mitochondria.

Free-soluble and outer membrane vesicle-associated VacA from Helicobacter pylori: Two forms of release, a different activity.

Helicobacter pylori releases VacA both as free-soluble and as outer membrane vesicle (OMV)-associated toxin. In this study, we investigated the amount of VacA released in each of the two forms and the role of each form in VacA-induced cell vacuolation in vitro. We found that: (1) free-soluble toxin accounted for about 75% of released VacA, while the remaining 25% was OMV-associated; (2) although OMV-associated VacA caused a statistically significant vacuolation, virtually all the vacuolating activity of a H.