Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems.

Plant pathogens are causing substantial economic losses and thus became a significant threat to global agriculture. Effective and timely detection methods are prerequisite for combating the damages caused by the plant pathogens. In the realm of plant pathogen detection, the isothermal amplification techniques, e.

Quadruplex and q-PCR based diagnostic assay to delineate the major quarantine and other seed-borne fungal pathogens of soybean.

Soybean is one of the most important crops grown worldwide and accounting for significant global trade including transgenic soybean. The crop is attacked by several seed-borne fungal pathogens and some of them are of quarantine concern for India. Keeping in view of the risks associated with movement of soybean seeds, sensitive and reliable molecular diagnostics have been developed for precise and simultaneous detection of three pathogens of quarantine concern for India namely, Diaporthe phaseolorum (stem blight), D.

Field and agroinoculation screening of national collection of urd bean () germplasm accessions for new sources of (MYMV) resistance.

Yellow mosaic disease (YMD) is a major problem in Urd bean ( L.) in India, which causes huge yield losses. Breeding for wide spectrum and durable (MYMV) resistance and cultivating resistant cultivars is the most appropriate and effective approach.

Draft genome sequence of ' Phytoplasma australasia', strain SS02 associated with sesame phyllody disease.

Phytoplasma' is an uncultivated, intracellular bacterial plant pathogen transmitted by phloem-feeding insect vectors. Among the group of phytoplasmas, the Peanut Witches' Broom or 16SrII group of phytoplasmas associated with various diseases cause severe crop losses every year in India. The '.

Expression analysis of and genes governing accumulation of provitamin-A, lysine and tryptophan during kernel development in biofortified sweet corn.

Traditional sweet corn possesses low levels of provitamin-A (proA), lysine and tryptophan. Mutant version of () gene affecting the accumulation of β-carotene (BC), β-cryptoxanthin (BCX) and proA, and () gene governing the enhancement of lysine and tryptophan were introgressed together into elite sweet corn inbreds through marker-assisted selection. Here, we analyzed the expression pattern of and genes among introgressed and traditional sweet corn inbreds at 20-, 24- and 28-days after pollination (DAP).