Leveraging SARS-CoV-2 Main Protease (M) for COVID-19 Mitigation with Selenium-Based Inhibitors.

The implementation of innovative approaches is crucial in an ongoing endeavor to mitigate the impact of COVID-19 pandemic. The present study examines the strategic application of the SARS-CoV-2 Main Protease (M) as a prospective instrument in the repertoire to combat the virus. The cloning, expression, and purification of M, which plays a critical role in the viral life cycle, through heterologous expression in in a completely soluble form produced an active enzyme.

The gram-negative bacterium as a model for testing the effect of carbonic anhydrase inhibition on bacterial growth.

Carbonic anhydrases, catalysing the reversible CO hydration reaction, contribute in all living organisms to the maintenance of stable metabolic functions depending on intracellular concentrations of carbon dioxide, bicarbonate, and protons. Recent studies have examined how CAs affect bacterial lifecycle, considering these enzymes druggable targets due to interference with their activities by using inhibitors or activators. Here, we propose cells as a model for testing the effect of acetazolamide (AZA), a potent CA inhibitor, on bacterial survival by evaluating growth through its glucose consumption.

Heterologous expression and biochemical characterisation of the recombinant β-carbonic anhydrase (MpaCA) from the warm-blooded vertebrate pathogen .

Warm-blooded animals may have on healthy skin, but changes in the skin microenvironment or host defences induce this opportunistic commensal to become pathogenic. Malassezia infections in humans and animals are commonly treated with azole antifungals. Fungistatic treatments, together with their long-term use, contribute to the selection and the establishment of drug-resistant fungi.

Inhibitory Effects of Sulfonamide Derivatives on the -Carbonic Anhydrase (MpaCA) from , a Commensal, Pathogenic Fungus Present in Domestic Animals.

Fungi are exposed to various environmental variables during their life cycle, including changes in CO concentration. CO has the potential to act as an activator of several cell signaling pathways. In fungi, the sensing of CO triggers cell differentiation and the biosynthesis of proteins involved in the metabolism and pathogenicity of these microorganisms.

Effect of amino acids and amines on the activity of the recombinant ι-carbonic anhydrase from the Gram-negative bacterium .

We here report a study on the activation of the ι-class bacterial CA from (BteCAι). This protein was recently characterised as a zinc-dependent enzyme that shows a significant catalytic activity ( 3.0 × 10 s) for the physiological reaction of CO hydration to bicarbonate and protons.