Computerized determination of proliferating cell nuclear antigen expression in meningiomas. A comparison with non-automated method.

Proliferating cell nuclear antigen (PCNA) expression has been proven to be a significant marker of cell proliferation in meningiomas, which correlates with growth rate and, as shown by several authors, possibly provides prognostic information concerning biologic behavior. However, the current method for determining PCNA labeling index (LI) is tedious and time consuming like all the nonautomated methods for evaluating cell kinetics, presenting high interobserver and interlaboratory variability and low reproducibility. In the present study, we introduce a semi-automated computer-assisted image analysis method for determining PCNA LI in 38 meningiomas, in parallel with the current nonautomated method.

Extraskeletal Ewing's sarcoma of the parotid region: a case report with the detection of the tumour's immunophenotypical characteristics.

A case report of a malignant small round cell tumour of the parotid region, with features of extraskeletal Ewing's sarcoma is presented. Besides its rare location in the soft tissues of the parotid area, the tumour expressed focal NSE and desmin immunoreactivity, indicative of multidirectional differentiation.

Ag-NORs in giant cell tumor of bones: are they useful in the estimation of tumor's behavior?

The number of nucleolar organizer regions (NORs) in giant cell tumors of bones was measured using the one-step silver staining technique (AgNOR) with black and white photographs and semiautomatic image analysis. The purpose of the study was to determine whether this method provides useful information on the biological behavior of bone GCTs. The number of AgNORS in bone CGTs (mononuclear cells: 3.

Echinococcosis of bone: clinico-laboratory findings and differential diagnostic problems.

Twenty cases of echinococcosis affecting the skeleton are reported. These cases were diagnosed at the Pathology Department of KAT General Hospital over a 12 year period (1976-1988). The parasitosis involved individual bones or skeletal parts (spine 6, pelvis 6, femur 4, tibia 1, scapula 2 and maxilla 1).

Follicular thyroid adenoma: signet ring cell variant.

An unusual case of a microfollicular adenoma of the thyroid gland with signet-ring cells in a 36-year-old euthyroid man is described. Mucin stains demonstrated the presence of mainly neutral glycoproteins with focal participation of acidic groups. Immunohistochemical staining for thyroglobulin was strongly positive.