Phosphatidylinositol kinase and phosphatidylinositol 4-phosphate kinase activities in Md 66 insect cell line.

Phosphatidylinositol kinase (PI kinase) and phosphatidylinositol 4-phosphate kinase (PIP kinase) activities in an insect cell line (Md 66) were studied by measuring the incorporation of 32P from [gamma-32P] ATP into phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) respectively. The apparent Km values of phosphatidylinositol kinase and phosphatidylinositol 4-phosphate kinase for ATP were 78.5 microM and 71.

Application of lipase to concentrate the docosahexaenoic acid (DHA) fraction of fish oil.

A commercial lipase preparation from Rhizopus niveus was used to concentrate the omega-3 fatty acid, docosahexaenoic acid (DHA) component in fish oil. The DHA content of cod-liver oil was 9.64% (w/w) of total fatty acids.

Fuel mobilization for energy metabolism in the silkmoth, Bombyx mori, during reproductive processes.

Flight muscles of male moth, B. mori seem to utilize carbohydrate preferentially as a source of energy for all its acrobatic movements during the search for female moth. Depletion of triacylglycerol from flight muscles without affecting its level from fat body suggests that this lipid fraction serves as a source of energy in flight muscles during insemination processes.