Intramolecular recombination R-triplex in solution: stabilization by bis-intercalator YOYO.

Recognition of double-stranded DNA with a mixed nucleotide sequence by oligonucleotide is a long-term challenge. This aim can be achieved via formation of the recombination R-triplex, accommodating two identical DNA strands in parallel orientation, and antiparallel complementary strand. In the absence of proteins the R-triplex stability is low, however, so that intermolecular R-triplex is not formed by three DNA strands in a ligand-free system.

[Properties of the Burkholderia pseudomallei insertional mutants deficient in membrane proteins production].

Transposon-induced B. pseudomallei mutants deficient in membrane proteins production were obtained for evaluation of the functional role of these cell components. In comparison with the wild type strain B.

[Cross-reacting antigens of pathogenic Burkholderia and some dangerous causative agents of infectious diseases].

Cross-reacting antigens in B. mallei, B. pseudomallei, B.

[A species-specific DNA probe for identifying toxic strains of anthrax pathogens].

On the plasmid DNA pOX01 of the anthrax pathogen two BamHI fragments were localized which facilitate detection of the Bacillus anthracis strains carrying pXO1 replicon. These fragments, after complete hydrolysis of plasmid DNA by HindIII, were cloned on the vector plasmids pUC19 and pBR322 by the "shot-gun" method in Escherichia coli cells. It is shown that the 900 bp BamHI/HindIII fragment from the pZAT1 recombinant plasmid has an ability for specific hybridization with DNA of toxigenic strains of B.