Publications by authors named "V B Skliadneva"

The conditions for linkage formation in phages Sd and P22 DNP under effects of sodium bisulfite were studied. Th linkage was estimated by the fall in phage infectiosity. After removal of the reagent excess prior to inoculation the modified phage was transferred to various buffers (12 salt systems).

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An interaction occurring between DNA cytosine and phage P22 protein was fixed using chemical modification with a nucleophilic agent, sodium bisulphite. Four minor proteins (P1, P20, P26, and P4) interacting with phage P22 DNA were isolated. Amino acids lysine and arginine were found to be the immediate partners of cytosine realizing this interaction.

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The structure of phage P22 DNA in situ was investigated by optical methods and by chemical modification with sodium bisulfite. On disruption of the phage particles by heating at 45 degrees a drop in absorbance at the 250 nm to 290 nm region was observed. At 260 nm this hypochromism was about 12%.

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A method is described for identification of amino acids participating in covalent DNA-protein cross-links formed in the course of modification of native CD phage by soldium bisulfite. The method is based on estimation of increased content of free amino acids, formed after treatment of the isolated cross-link products using equimolar mixture of O-methyl hydroxylamine and sodium bisulfite. Thin layer chromatography on the plates Fixion 50 x 8 was used for comparative semi-quantitative analysis of amino acids.

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The interaction between sodium bisulfite and the cytosine residues within the intraphage DNA of phage SD was studied to elucidate the structure of viral nucleoprotein. Hydrolysis with perchloric acid of bisulfite-modified phage SD results in 18% decrease of cytosine and appearance of products having the properties of cytosyl amino acids (most probably cytosyl lysine). When the modified phage before hydrolysis was subjected to mild destruction in 0.

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