[The mechanism of metabolic denitration of nitroheterocyclic compounds].

It has been found that denitration of nitroheterocyclic drugs (NHCC) in mouse liver homogenate is one of the pathways of their biotransformation. This process is accompanied by the release of NO and NO-heme iron complex formation. The yield of complex increases linearly with time of NHCC incubation with liver homogenate and depends on NHCC structure.

[Computer analysis of anisotropic ESR spectra and the structure of radical H and OH adducts of nucleic acid components in frozen aqueous solutions].

Computer analysis of ESR spectra recorded under gamma-irradiation of polycrystal and glass-like solutions of nucleotides and nitrous bases was carried out to determine the localization sites of free valency (damage) in DNA macromolecule when interacting with water radiolysis products (OH and H). The analysis was performed on a mini-computer with the program of reconstruction of ESR anisotropic spectra in the variation search regime. When calculating the theoretical spectra anisotropic width of the lines, g-factors and HHS constants on X and Z axes were taken into account.

[Determination of the nitrogen-fixing activity of Rhizobium japonicum under sterile microvegetative conditions].

The nitrogen fixing activity of Rhizobium japonicum in symbiosis with soya grown in the sterile microvegetative conditions at an air humidity of 70%, at a temperature of 20 degrees C and a length of light day of 16 hours was assayed using the acetylene technique. The plants were cultivated in phytotron in glass tubes (245 cm3) illuminated with xenon lamps. This technique can be used, apparently, to determine the nitrogen fixing activity of other legumes and cereals.