[Use of serotonin for treatment of patients with diabetic angiopathies].

In 26 patients (female 11, males--15) with diabetic angiopathy (necrosis of the foot or toes) local measurement of pO2 by transcutaneous mode was carried out before and during introduction of serotonine adipinate. Simultaneously with evaluation of pO2 in 14 patients electromyography (EMG) was made. Serotonine adipinate was introduced intravenously with a speed of 5.

[Neurologic disorders in patients with Madelung's lipomatosis].

The authors had 21 patients with a rare disease, Madelung's lipomatosis. Vegetative disorders of segmental and suprasegmental levels were revealed in all patients. The character of neuropathies in Madelung's lipomatosis is discussed in detail.

[Changes in the human brain and spinal cord after short-term cardiac arrest and prolonged respiratory resuscitation].

Clinicomorphological data on 12 cases of long-term APV (up to 35 days) are reported. The degree of morphological changes was found to vary with CNS compartment. The authors emphasize clinicomorphological alterations in the spinal cord, particularly in the cervical segments, where almost complete absence of cells and reactivity of glial elements were observed.

[An electron radioautographic study of the dynamic decrease in the level of RNA synthesis in human brain cells at different times after death].

The above study is performed in neurons, oligodendrocytes and capillary endotheliocytes of human brain 4-13 hours after death. Brain biopsies after the surgery and early necropsies served as the material. RNA synthesis in some neurons and glial cells is retained to some degree 8-13 hours after death.

[Amplification of DNA for 15-30 minutes in single-use pipette tips].

Amplification of 200-1000-bp DNA fragments was performed in 15-30 min using a rapid thermal cycler based on the commercial instrument TC-1000-1 (IRLEN, St. Petersburg, Russia). Plastic pipette tips were used as thin walled, high surface to volume-ratio tubes, to increase the rate of heating (cooling) of 20 microliters samples, which allowed the time required for DNA amplification to be considerably reduced (5-10 times).