LDL apheresis with dextran sulfate and angiotension receptor antagonist (Losartan).

Anaphylactoid reactions during low-density lipoprotein (LDL) apheresis with dextran sulphate adsorption have been reported in patients taking angiotensin converting enzyme (ACE) inhibitors.

Occurrence of multiple aberrantly spliced mRNAs of the LDL-receptor gene upon a donor splice site mutation that causes familial hypercholesterolemia (FHBenevento).

A novel point mutation of the LDL-receptor gene was found in an Italian patient with homozygous familial hypercholesterolemia. The SSCP analysis of the promoter and of 16 out of the 18 exons of the LDL-receptor gene was negative, suggesting that the mutation might be located in the region of the gene encompassing exons 14 and 15, a region that had not been amenable to polymerase chain reaction (PCR) amplification from genomic DNA. This region was amplified from cDNA by reverse transcription PCR (RT-PCR).