Glycolysis is a main catabolic pathway of glucose metabolism, accompanied by ATP synthesis. More than 30 enzymes are involved in glycolysis, and genes that encode them can be considered housekeeping genes due to the high conservatism and evolutionary antiquity of the process. We studied the expression of these genes in kidney papillary cancer and planocellular lung cancer via the bioinformatic analysis of transcriptome database and method of quantitative real time PCR.
View Article and Find Full Text PDFFluorescent 11-[(9'-anthracenyl)methoxy]undecyl phosphate and P1-{11-[(9'-anthracenyl)methoxy]undecyl}-P2-(alpha-D-galactopyranosyl) diphosphate were chemically synthesized for the first time. The ability of the first compound to serve as substrate-acceptor ofgalactosyl phosphate residue and the second compound of mannosyl residue in enzymic reactions catalyzed by galactosylphosphotransferase and mannosyltransferase from Salmonella newport membrane preparation was demonstrated.
View Article and Find Full Text PDFP(1)-(Phenoxyundecyl)-P(2)-(alpha-D-galactopyranosyl) diphosphate as well as P(1)-(11-phenoxyundecyl)-P(2)-(alpha-D-glucopyranosyl) diphosphate are newly synthesized and their ability to serve as substrate-acceptor of mannosyl residue in enzymic reaction catalyzed by mannosyltransferase from Salmonella newport membrane preparation is investigated. The possibility ofgalactosyl-containing derivative to serve as mannosyl acceptor from GDP-Man is established whereas glucosyl-containing compound is inactive in this process.
View Article and Find Full Text PDFRadiats Biol Radioecol
November 2012
Some physical treatments, such as microwave- and gamma-radiation and magnetic field, induce long-term transition of air-dry seeds from the fraction of strong seeds into the weak seed fraction, due to non-enzymatic hydrolysis ofbiomacromolecules. These physical factors make water molecules more active, which is followed by the release of water molecules from the hydration layer, disturbance of this layer structure, further activation of water molecules by means of the "domino effect," and accumulation of hydrolysis products.
View Article and Find Full Text PDFRadiats Biol Radioecol
November 2012
The seeds with low level of room temperature phosphorescence (RTP) were selected from a lot of air-dry peas (Pisum sativum) with 62% germination. These strong seeds (95-97% germination percentage) in air-dry, imbibed or emerged states were exposed to 905 MHz GSM-band electromagnetic radiation (EMR). The following effects of EMR were observed.
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