[Growth stimulating, fungicidal and nematicidal properties of new microbial substances and their impact on si/miRNA synthesis in plant cells].

New substances on the basis of soil streptomycete Streptomyces avermitilis metabolites and also compositions of preparation averkom with elicitors show growth regulating activity and bioprotective properties, proved in experiments with wheat spring of variety Grizo and cucumbers of variety Nezhinskiy. Most of the created substances possess the antagonistic action in relation to phytopathogenic fungi, as well as antinematode activity in relation to the gallic nematode Meleodoidyne incognita. In the conditions of the artificial nematode infecting the preparation averkom and its modifications demonstrate protective action (up to 85-100 %) on cucumber sprouts of Nezhinskiy variety.

[Isolation and amplification cDNA of 8H07 gene conservative region of nematode Heterodera schachtii with high relationship to its rape homolog].

Original method of small regulatory si/miRNA isolation from plant cells was elaborated. PCR amplification of fragment cDNA 8H07 nematode Heterodera schachtii gene was carried out. Using Northern-blot method hybridization of plant si/miRNA with cDNA fragment of conservative region 8H07 gene the presence of their high homology is found out.