[Phosphoinositide breakdown and diacyl glycerin formation in human thrombocytes as affected by a lipopolysaccharide toxin].

The interaction of lipopolysaccharide toxin (LPS) with isolated washed human blood platelets has been studied. LPS was found to induce the rapid (1-2 min) and marked (15-20%) breakdown of mono- and polyphosphoinositides and formation of significant amounts of diacylglycerols (ca. 20%).

[Ability of a lipopolysaccharide toxin to intensify the functional response of human thrombocytes to thrombin stimulation].

The preincubation of isolated washed human platelets with lipopolysaccharide toxin (LPS) from Salmonella typhimurium (10 min, 37 degrees) speeds up the thrombin-induced aggregation. Besides, LPS-pretreatment was shown to alter the turnover of polyphosphoinositides stimulated by thrombin and significantly elevated concentrations of diacylglycerol and thromboxane B2 in comparison with control platelets. However, LPS does not influence on the lipid fluidizing effect of thrombin action as testifies by ESR spectroscopy with doxyl-stearic acids as spin-probes.

[Effect of divalent cations on the properties of NaCl-stimulated ATPase activity in the mucosa of the rabbit small intestine].

The effect of bivalent cations on NaCl-stimulated ATPase activity in rabbit small intestinal mucosa was studied. Unlike Ba2+ and Zn2+, Ca2+ and Mg2+ added to the incubation medium at a concentration of 0.25 mM completely change ATPase activity.