Essential edema-proteinuria-hypertension (EPH) gestosis still represents an important obstetrical problem. We have investigated the activity of carboxypeptidase H (CPH), phenylmethylsulfonyl fluoride inhibited carboxypeptidase (PMSF-CP), carboxypeptidase M (CPM) and angiotensin-converting enzyme (ACE), the main carboxypeptidases in human placenta under normal conditions and mild EPH-gestosis. Gestosis was accompanied by the decrease in activity of the enzymes involved into metabolism of regulatory peptides (ACE, CPH, PMSF-CP, CPM) compared with their activity in placenta under physiological pregnancy.
View Article and Find Full Text PDFThe effect of a single atropine administration on the activities of angiotensin-converting enzyme and carboxypeptidase N taking part in peptides metabolism was studied. Changes in the enzymes activities after administration were evident during at least 72 h. The role of these enzymes in the metabolism of neuropeptides under the effect of atropine was discussed.
View Article and Find Full Text PDFEffect of a single emotional-algesic stress on the carboxypeptidase H and PMSF-inhibited carboxypeptidase activities (phenylmethylsulphonilfluoride-inhibited carboxypeptidase) taking part in the final stage of formation of biologically active neuropeptides from precursors, was studied. Activity of the enzymes depended on duration of the stress and time after the stress in pituitary and adrenal glands. Differences in changes of carboxypeptidase H and PMSF-inhibited carboxypeptidase activities was found, especially in adrenals.
View Article and Find Full Text PDFIt is revealed, that the activity of neuropeptide metabolism enzymes (carboxypeptidase H, phenylmethylsulfonilfluorid-inhibited carboxypeptidase) in the female rat tissues depends upon the stage of estrus cycle. The carboxypeptidase H activity in the pituitary gland is the highest in proestrus; it is almost 3 times higher in comparison with diestrus; it is a little bit higher in striatum on the stage of estrus, than in diestrus and proestrus, in adrenals on the stage of proestrus and estrus it is a little bit lower, than in diestrus; in the ovaries on the stage of proestrus it is much higher, than in estrus and diestrus. The activity of PMSF-inhibited carboxypeptidase in ovaries on the stage of proestrus and diestrus is 1.
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