[Disordered regions in C-domain structure of influenza virus M1 protein].

Influenza virus matrix M1 protein is one of the main structural components of the virion performing also many different functions in infected cell. X-ray analysis data with 2.08 angstrom resolution were obtained only for the N-terminal part of M1 protein molecule (residues 2-158) but not for its C-terminal domain (159-252).

[The concept of transmembrane asymmetry of lateral domains in biomemranes and influenza virus envelope fine structure].

The molecular architectures of enveloped viruses are one demonstrative example of perfectly arranged macromolecular complex that is achieved through the structural specificity of virus assembly. Virus morphogenesis is a multi-step process that depends on the concerted actions of many viral and cellular components as well as fitted organization of main viral constituents. Viral envelope was shown to be composed of the mixture of lipid raft and non-raft domains.

[Determination of concentration and aggregate size in influenza virus preparations using the true UV-absorption spectra].

It is well-known that influenza virus (IV) preparations are characterized by very large contribution of light-scattering to their UV absorption spectra. With the help of so called extrapolation method we managed to measure true absorption spectra of IV preparations and to determine absorption coefficients (E0.1(1) (cm, 280)) for the intact IV virions and for IV subviral particles.

[Dependence of kinetic constants of intact human lymphocyte beta2-adrenoreceptors on the nature of the radioligands used].

An analysis of human peripheral blood intact mononuclear lymphocyte beta 2-adrenoreceptors showed that the hydrophilic radioligand 3H-CGP-12177 binds at various temperatures only to surface receptors. The density of receptors as determined by the binding of the lipophilic antagonist 125I-CYP at 37 degrees C is 2 times as high as that at 4 degrees C. The affinity of lymphocyte beta 2-adrenoceptors for 1-isoproterenol measured by the labeled ligand displacement at low (4 degrees C) temperatures is by two or three orders of magnitude higher than that at 37 degrees C.

Beta-blocker action on lymphocyte proliferative response in essential hypertension.

The proliferative response of phytohaemagglutinin (PHA) - stimulated lymphocytes of healthy donors and hypertensive subjects was analysed according to 3H-thymidine incorporation and cell cycle phase position on a flow cytofluorimeter. 3H-thymidine uptake and the percentage of cells in (S + G2) phase were significantly lower in hypertensive patients. After short-term propranolol administration the lymphocyte blastogenic response in essential hypertension increased by 30--100%.