[Mercury ions inhibit human neutrophil apoptosis induced by ultraviolet C irradiation].

The action of ultraviolet C (UVC) and mercury ions (HgCl2) on human neutrophil apoptosis was investigated by flow cytometry. It is shown that HgCl2 (5-10 microM) inhibits UVC-dependent acceleration of neutrophil apoptosis but does not inhibit spontaneous apoptosis.

[Apoptosis dynamics in human neutrophils induced by ultraviolet C irradiation during lipopolysaccharide and zinc exposure].

The action of ultraviolet radiation with lambda = 254 nm (UVC), zinc and lipopolysaccharide on the apoptosis of human neutrophils was investigated by flow cytometry. It was shown that zinc (0.2-1 mM) inhibits the UVC-dependent acceleration of neutrophil apoptosis.

[Effect of urea on conformational changes in the active site of native and reconstituted Ca-ATPase of the sarcoplasmic reticulum].

The effect of urea (1-3 M) on conformational changes in the active center of native and reconstituted Ca-ATPase of sarcoplasmic reticulum modified by fluorescein-5-isothiocyanate (FITC) was studied using the method of fluorescence titration by neodymium (Nd3+) ions. Based on the analysis of curves of fluorescence quenching of FITC-labeled Ca-ATPase by Nd3+ ions, the parameters characterizing the structural changes in the Mg-ATP binding center were determined. It was assumed that FITC and Nd3+ ion bind to different polypeptide fragments moving relative to each other, which provides the conformational lability of the nucleotide binding site at some stages of the catalytic cycle.

Steroid-induced conformational changes of FITC-labelled sarcoplasmic reticulum Ca2+-ATPase.

Interactions between transmembrane and cytoplasmic domains of Ca2+-ATPase from sarcoplasmic reticulum (SR) have been studied. To affect the hydrophobic transmembrane domain, we used four amphiphilic steroids - esters of a dibasic acid and 20-oxypregnene. All four steroids contained cholesterol-like nuclei and differed by the structure of side chains.