[Peculiarities of detection of 4-nitro-3-(trifluoromethyl)-aniline in the biological material].

The objective of the present work was to study peculiarities of detection of 4-nitro-3-(trifluoromethyl)-aniline in the biological material with the use of TLC, GC-MS, and electron spectrophotometry. We have proposed the rationale for the application of acetone as an insulating agent for the extraction of 4-nitro-3-(trifluoromethyl)-aniline from the cadaveric hepatic tissue and biological fluids. It was shown that this compound is possible to separate from endogenous biomaterials on the silicagel L column (40/100 mcm).

[Peculiarities of isolation of 4-nitroanilin from the biological material].

The objective of the present work was to elucidate the peculiarities of isolation of 4-nitroanilin from the biological material with the use of TLC and HPLC techniques, low-pressure column chromatography, and spectrophotometry. The results of the study made it possible to substantiate the application of 1,4-dioxane as an agent for the isolation of 4-nitroanilin from the biological material. The optimal conditions of the procedure for isolation of 4-nitroanilin from the hepatic tissue using 1,4-dioxane were determined.

[Extraction of flutamide from aqueous solutions].

The objective of the present study was to elucidate peculiarities of flutamide extraction from aqueous solutions using he liquid-liquid extraction technique and spectrophotometry in the visible spectrum. The results of flutamide extraction were shown to depend on the chemical nature of the extractant, pH of the aqueous phase as well as its saturation with water and electrolytes. The study has demonstrated that the optimal conditions for flutamide extraction by ethylacetate are created by using the aqueous phase with the pH values in the range from 1.

[Population genetic differentiation of white-spotted char Salvelinus leucomaenis (Pallas) of Russian Far East].

The genetic structure of the populations in white-spotted char Salvelinus leucomaenis (family Salmonidae) was determined based on variations in ten microsatellite DNA loci in samples from different parts of the species range in the Russian Far East. In a number of samples, variations in 21 allozyme loci were examined, of which five loci were found to be polymorphic. The overall diversity level in the examined markers was comparable to that observed in a closely related char species, Dolly Varden.

[The distribution of 4-nitroanilin in the body of warm-blooded animals].

Specific distribution patterns of 4-nitroanilin in the body of warm-blooded animals (rats) were studied after the in vivo administration of this poisonous substance. It was shown that unmetabolized 4-nitroanilin accumulates in large quantities in the internal organs of the intoxicated animals. Its highest levels are found in the stomach and its contents, kidneys, and lungs.