[Immunophenotypic heterogeneity of CD7+CD4-CD8--acute leukemia].

Fourteen cases of lymphoid and myeloid acute leukemia (AL) were studied for expression on blast cells of CD7 antigen, a cell surface marker found early during T lineage differentiation. This heterogenic group of CD7+ CD4-CD8- AL includes distinct cytological subvariants with: myeloid (AML MO, M1, M4, M5) and lymphoid (pre-T-cell) commitment, biphenotypic or mixed lineage AL and AL with minimal signs of blast cell differentiation, which appear not to follow lineage restriction. The latter subset of AL may represent the transformed counterpart of an early stem cell prior to lineage commitment.

[Immunoenzyme cytochemical methods, monoclonal antibodies and lectins in the diagnosis of tumors of the lymphoid tissue in children].

ICO, IPO and LT series monoclonal antibodies, lectins, PAP and APAAP methods were used to study blood, bone marrow and lymph node cells in children with acute lymphoblastic leukemia (ALL), non-Hodgkin's malignant lymphomas and Hodgkin's disease. The immunological phenotype of malignant lymphoid cells has been characterized and cytological variants of ALL and lymphomas of T and B cell origin have been distinguished.

[Study of lymphocyte subpopulations using peanut, soybean and snail lectins].

The method of penning in plastic dishes was to isolate the PNA+ and PNA- cell populations from the tonsil tissue. Carbohydrate determinants of lymphoid cell surface membranes being at varying stages of antigen-independent and antigen-dependent differentiation have been studied with the use of peanut, soya and edible snail lectins conjugated with horse radish peroxidase.

[Use of monoclonal antibodies and lectins in the study of subpopulations of lymphoid cells in normal state and in leukemia].

The heterogeneity of cell populations with peanut agglutinin receptors in reactive tonsilar tissue has been stated. It contains B lymphocytes (HLA-DR+, CD 10+, IPO-10+ cells), part of T-cells (CD 4+), but no CD 8+ lymphocytes. PNA+ CD 10+ cells are the normal analogues of centrocytic non-Hodgkin's lymphomas.

[Immunocytochemical methods using monoclonal antibodies and lectins in the diagnosis of lymphoblastic leukemia in children].

ICO, IPO, LT monoclonal antibodies and lectins were used to study blood cells in 18 children with acute lymphoblastic leukemia (ALL). The immunological phenotype has been characterized, and two cytological variants of ALL of T-cell nature, and four variants of ALL of B-cell origin have been distinguished, differing by the degree of blast cell maturity.