Changes in neuropil ultrastructure in hippocampal field CA1 in rat pups after application of hyaluronidase.

Electron microscopy and electrophysiological studies were performed on cross-sectional slices of the hippocampus of four-week-old male rat pups (n = 35) to detect ultrastructural changes in hippocampal field CA1 and the characteristics of the formation of excitatory postsynaptic potentials in this area of the brain after incubation of slices in hyaluronidase solution (10 U/ml), whose specific substrate is the extracellular matrix glycosaminoglycan hyaluronic acid. At 1.5 min after enzyme application, there were reductions in synaptic cleft widths in axodendritic contacts of the striatum radiatum of hippocampal field CA1 by 15-25%, which were consistent with increases seen in the amplitudes of excitatory postsynaptic potentials.

[Changes of the neuropil ultrastructure in the CA1 hippocampal area of rat pups after hyaluronidase treatment].

Electron microscopical and electrophysiological studies were carried out in cross-sections of the hippocampus of 4-weeks-old male rat pups (n = 35) to detect the ultrastructural changes in CA1 hippocampal area and the peculiarities of excitatory postsynaptic potential formation in this brain area after the incubation of the sections in the solution of hyaluronidase (10 U/ml), the enzyme which specifically degrades the extracellular matrix glycosaminoglycan--the hyaluronic acid. The reduction of the width of synaptic cleft by 15-25% in the axo-dendritic contacts of the stratum radiatum of CA1 hippocampal area was detected 1.5 min following the application of enzyme, coinciding with the increase of the excitatory postsynaptic potential amplitude.

Dependence of electrical activity of neurons in rostral parts of spinal trigeminal nucleus on functional state of trigeminal Gasser ganglion.

Unilateral injection of 100 microl 1% lidocaine into the trigeminal Gasser ganglion of narcotized rats produced a long-term moderation of the discharge rate of neurons in the ipsilateral (relative to the side of injection) rostral area of the spinal trigeminal nucleus. Activity of neurons in the contralateral rostral area of the spinal trigeminal nucleus was not blocked. Functional state of neurons in the trigeminal ganglion determines discharge activity of ipsilateral neurons of the spinal trigeminal nucleus.

[Outpatient care for urological patients].

The presence of adequately equipped surgical unit in outpatient clinics provides surgical aid to more patients with urological diseases. Preoperative preparation, aseptic and antiseptic measures, local anesthesia were routine. 64 operations for phimosis, condylomatous growth of the foreskin, hydrocele, cyst of the epididymis and spermatic cord, chronic epididymitis, short frenulum of the prepuce were performed in outpatient setting.