Excessive Adventitial and Perivascular Vascularisation Correlates with Vascular Inflammation and Intimal Hyperplasia.

Albeit multiple studies demonstrated that (VV) have a crucial importance in vascular pathology, the informative markers and metrics of vascular inflammation defining the development of intimal hyperplasia (IH) have been vaguely studied. Here, we employed two rat models (balloon injury of the abdominal aorta and the same intervention optionally complemented with intravenous injections of calciprotein particles) and a clinical scenario (arterial and venous conduits for coronary artery bypass graft (CABG) surgery) to investigate the pathophysiological interconnections among VV, myeloperoxidase-positive (MPO) clusters, and IH. We found that the amounts of VV and MPO clusters were strongly correlated; further, MPO clusters density was significantly associated with balloon-induced IH and increased at calciprotein particle-provoked endothelial dysfunction.

[Ultrastructure of neointima of native and artificial elements of the blood circulatory system].

Objective: To compare the neointima structure in conduits for coronary bypass grafting, bioprosthetic heart valves, tissue-engineered vascular grafts, and metal stents.

Material And Methods: The objects of the study were the fragments of the human internal thoracic artery, experimental biodegradable vascular prostheses, leaflets of xenopericardial bioprostheses of heart valves, and fragments of stented vessels. Tissue samples were fixed in formalin and post-fixed in osmium tetroxide.

EMbedding and Backscattered Scanning Electron Microscopy: A Detailed Protocol for the Whole-Specimen, High-Resolution Analysis of Cardiovascular Tissues.

Currently, an ultrastructural analysis of cardiovascular tissues is significantly complicated. Routine histopathological examinations and immunohistochemical staining suffer from a relatively low resolution of light microscopy, whereas the fluorescence imaging of plaques and bioprosthetic heart valves yields considerable background noise from the convoluted extracellular matrix that often results in a low signal-to-noise ratio. Besides, the sectioning of calcified or stent-expanded blood vessels or mineralised heart valves leads to a critical loss of their integrity, demanding other methods to be developed.

Effect of Hydrogen Sulfide on Deformability of Rat Erythrocytes.

The influence of a hydrogen sulfide donor NaHS (2×10-10 M) on the rat erythrocyte deformability was analyzed by laser diffractometry. NaHS (6×10 M) increased, while NaHS (10 M) reduced erythrocyte deformability. The effect of NaHS (6×10 M) was similar to that of NO donor sodium nitroprusside (SNP, 10 M).

Synthesis, Redox Properties and Antibacterial Activity of Hindered Phenols Linked to Heterocycles.

A series of benzotriazole, cyclic amides and pyrimidine derivatives, containing 2,6-di--butyl-phenol fragments, were synthesized. The redox properties of obtained compounds were studied using the cyclic voltammetry on a platinum electrode in acetonitrile. The oxidation potentials of all substances were comparable to those of BHT.