[Hyperbaric oxygenation in the treatment of facial neuritis].

The effectiveness of hyperbaric oxygenation (HBO) in a multiple modality therapy versus the conventional treatment of patients with facial neuritis was investigated. HBO was used in the treatment of 42 patients. Twenty-nine patients with a similar picture of the diseases treated by conventional methods without the use of HBO constituted the control group.

[Regulatory region of fr phage replicase cistron. II. Isolation and structure of specific fr RNA fragments].

A new set of short RNA templates has been prepared for functional studies in initiation of translation in vitro. Number of individual RNA fragments which contain complete or part of the initiatory region of phage fr replicase cistron were isolated from complex fr RNA--fr coat protein. Their primary structure were determined by using standard fingerprint technique and rapid gel sequencing.

The regulatory region of MS2 phage RNA replicase cistron. III. Characterization of fragments resulting from S1 nuclease digestion.

The functionally active fragments MS2 R(-53 leads to 6) and MS2 R(-53 leads to 3) comprising the regulatory region for the replicase cistron have been isolated from MS2 RNA-coat protein complex following T1 RNase digestion. In order to obtain shorter fragments, active in coat protein binding and initiation of translation, MS2 R(-53 leads to 6) was cleaved with S1 nuclease. The results indicate that S1 nuclease attacks the most susceptible loop regions of the two hairpin helices of MSZ R(-53) leads to 6).

[Regulatory region of the MS2 phage RNA replicase cistron. Accesibility of MS2 RNA specific fragment to T1 RNAse digestion and chemical modification with kethoxal].

Partial digestion with T1 RNAase and chemical modification with kethoxal were used to study stability of two hairpin in the proposed secondary structure of the functionally active MS2 RNA fragment MS2 R(--53 leads to 6), containing the regulatory region of the phage replicase cistron. Analysis of the products obtained after the above treatments showed that T1 RNAase and kethoxal attacked predominantly the guanosine residues in the hairpin b of the MS2 R(--53 leads to 6). This implies that in contrast to the structurally stable hairpin a of the polynucleotide, hairpin b appears to be more labile and may exist under the present experimental conditions in equilibrium with its open form.