[Rotavirus etiology of hospital cases of acute gastroenteritis].

The etiology of enteric illnesses occurring during hospitalization in children admitted in January-May, 1985, for respiratory tract illnesses was studied by several methods including electron microscopy (EM), enzyme immunoassay (EIA), and PAG electrophoresis. Rotaviruses were detected in 22 (44.9%) t of 49 children with symptoms of intestinal infection, in February in 11 (84.

[Isolation of immunoglobulins from the egg yolk of hens immunized with intestinal viruses].

Antibodies from the yolk of eggs of chicken immunized with enteric viruses (simian rotavirus SA 11, poliomyelitis virus type 2, and Coxsackie B2) were obtained. The time course of the emergence and amplification of antibody in the yolk was followed for several months by neutralization tests and enzyme immunoassay. The long-term persistence of a high antibody level in the yolk, the simplicity of generation of large amounts of chromatographically pure preparations, as well as specificity of chicken immunoglobulins open ways for their employment in different test systems for the detection and identification of enteric viruses.

[Discovery and quantitative determination of intestinal viruses by the detection of viral antigens in infected cells using an immunoperoxidase method].

The use of immunoperoxidase method for detection of virus antigens in a fixed infected cell monolayer on microplates (cellular immunoperoxidase method, CIPM) for poliomyelitis and hepatitis A virus as well as for human and simian SA II rotaviruses is described, as well as the method of quantitation of rotaviruses by counting of virus multiplication foci. The CIPM combines the advantages of the methods based on the infectivity determinations and of immunochemical methods, and may be used both for diagnosis and for detection and quantitation of enteric viruses.