[Changes in the myeloperoxidase and acid phosphatase activities in human peripheral blood neutrophils with the cells stimulated in vitro].

In four series of experiments human peripheral blood neutrophils were found capable of synthetizing the active forms of such enzymes as myeloperoxidase and acid phosphatase after stimulation with opsonized zymosan, and the optimum conditions for testing the synthetizing activity of neutrophils were established. The examination of 39 practically healthy donors revealed an approximate equilibrium between the synthesis of the active forms of the enzyme and its excretion from the cell after this cell was activated. Considerable changes in the enzymatic activity of peripheral blood neutrophils were found in patients with yersiniosis, chronic herpes, chronic bronchitis and AIDS-associated complex.

[Enzyme indicator of the immunobiologic activity of antigens].

The test-system has been elaborated for the determination of the ability of different antigens in the cultures of the mouse peritoneal macrophages. Measuring an intracellular acid phosphatase activity, reflecting the extent of the cell activation, was taken as a principle of this test-system. With the help of this test-system ability of a few antigens (lipopolysaccharide, polysaccharide, glycoprotein, protein and low molecular weight substances--peptide and glycopeptide) have been studied.

[Elastase and acid phosphatase activity in bronchoalveolar lavage fluid during chronic bronchitis].

The dynamics of acid phosphatase and granulocytic elastase activities in bronchoalveolar washings of 40 patients with chronic catarrhal bronchitis was studied. In case of effective cure, the activities of these enzymes were increased shortly on the 10-13 days from beginning of the cure. In order to study the nature of this enzymes, the additional researches were conducted.

[The rapid diagnosis of chronic nonspecific lung diseases].

Rapid methods for the detection of viral antigens and immunoglobulins in nasal and bronchial washings from patients with chronic nonspecific pulmonary diseases (CNPD) are described. These methods are based on viral antigen and immunoglobulin agglutination with cellulose particles sensitized with specific sera and gamma-globulin fractions. The investigation takes just 3-5 min.

[Immunologic assessment of preparations of the RS virus at the stages of its purification and concentration].

The work demonstrates the immunogenic potency of some preparations of RS virus, differing in the degree of their purification and introduced by multiple intranasal administration. The immunogenic potency of these preparations was manifested by the synthesis of antibodies determined in the coagglutination test (the specificity of these antibodies was confirmed in the reaction of the neutralization of RS virus), as well as by sensitization determined in the leukocyte migration inhibition test. Immunization with concentrated virus was more effective than immunization with the preparation of virion fraction.