[Obtaining human recombinant (serine-17) beta-interferon by the method of oligonucleotide-directed mutagenesis and its expression in Escherichia coli].

The gene of human mutant (serine-17) fibroblast interferon was isolated with the use of highly efficient oligonucleotide-directed mutagenesis. On the basis of the constructed expression plasmid pPR-IFN Ser17 a strain producing human mutant beta-interferon (VKPM V-4678) was developed. It was shown that the specific activity of the human mutant (serine-17) fibroblast interferon was 1 order of magnitude higher than that of the recombinant interferon which reaches the specific activity of natural fibroblast interferon.

[Enterosorption in the treatment of patients with chronic urticaria].

A synthetic carbon sorbent (SKNP-2) was included in the treatment of 197 patients with relapsing urticaria. Courses lasted 7, 14, 20 days. The clinical effect was favourable and its efficacy increased with prolongation of the therapy course time using the enterosorbent.

[Changes in the rosette-forming capacity of peripheral blood lymphocytes in patients with ischemic heart disease after administration of atherogenic lipoproteins].

Low-density lipoproteins, isolated from the serum of patients with hypercholesterolemia (dislipoproteinemia, type IIa), increased significantly the number of active and recovered rosette-forming cells (RFC) at incubation with lymphocytes of coronary patients. The use of lipoproteins, belonging to all atherogenic classes, did not essentially alter lymphocyte ability to form total E-RFC. Purified apolipoprotein B reduced lymphocyte capacity for active rosette formation.

[Characteristics of the action of modified atherogenic-class lipoproteins on the functional activity of peripheral blood lymphocytes in patients with ischemic heart disease].

In a study of the effect of lipoproteins of atherogenic classes on proliferative activity of peripheral lymphocytes in patients (n = 44) with ischemic heart disease (IHD) and healthy subjects (n = 16), VLDL isolated from hyperlipidemia blood were shown to have a more suppressive effect on proliferation of PHA-induced lymphocytes than those from healthy subjects. Effect analysis of VLDL isolated from patients with different hyperlipidemia types revealed that VLDL obtained from high triglyceride plasma depressed proliferation 85-75%, while VLDL withdrawn from high cholesterol plasma 45-55% (p less than 0.05).

[Absorption of immune complexes by neutrophils in patients with ischemic heart disease].

Neutrophil absorption capacity was studied in 45 coronary patients, using immune complexes, containing various immunoglobulin classes. Neutrophil capacity for absorption increases at early stages of the disease and decreases with the progress of its duration and severity, while serum immune complex levels go up.