[Sequential staining of the polymorphic regions of human acrocentric chromosomes using different cytochemical methods].

The chromosome preparations of 12 normal individuals were consequently stained with four different techniques (RV, Q, C and Ag). Correlations between morphological and staining peculiarities of specific polymorphic segments of the short arms of the same acrocentrics were investigated. It was determined that except correlations for the length of satellite stalks and the size of Ag-deposits, the polymorphic signs revealed with Q-, C- and Ag-techniques appear to be independent and not correlating with each other.

[Comparative analysis of the population polymorphism of human silver-staining chromosomal nucleolus organizer regions].

The population study of nucleus organizing regions (NOR) activity in individuals of Georgian nationality was carried out using the Ag-method. The results obtained were compared with those for other populations: Russian, Viena-Ulm, Estonian etc. The mean number of active NORs for Georgians is 9.

[Sensitive method of detecting 5-bromodeoxyuridine differentially incorporated into the chromosomes of mammals].

A modified method for fluorochrome staining with acridine orange of chromosome preparations for revealing the BUdR label is described. It has been shown that the alkaline medium used increases the contrast between chromosome areas with varying BUdR content and photoresistance of the staining.

[Genetic determination of the activity of nucleolar-organizing regions of human chromosomes].

Activity of nucleolar organizer regions (NORs) was studied in cultured blood lymphocytes of 8 monozygotic and 8 dizygotic twin pairs using Ag-staining of acrocentric chromosomes. These chromosomes were identified by G-banding. NOR activity was estimated by the number of Ag-stained regions, their distribution among individuals acrocentrics and the size of silver deposits.

[Quantitative assessment of human chromosomal polymorphism with regard to paracentromeric heterochromatin].

The measurement of C-segment length in chromosomes 1, 9 and 16 of 7 individuals was carried out. The regression analysis was employed to study a change of the C-segment sizes in the process of mitotic chromosome condensation. Typical values of C-segment length for chromosomes 1, 9 and 16 are about 1.