Effects of the combination of chitosan and Acinetobacter KU011TH on the growth and health performances and disease resistance of juvenile hybrid catfish (Clarias gariepinus × C. macrocephalus).

Aquatic animal health management has become a crucial component in the goal of increasing catfish aquaculture productivity. Additionally, hybrid catfish (Clarias gariepinus × C. macrocephalus) has been promoted as a highly profitable freshwater fish in Asia.

Overcoming taxonomic challenges in DNA barcoding for improvement of identification and preservation of clariid catfish species.

DNA barcoding without assessing reliability and validity causes taxonomic errors of species identification, which is responsible for disruptions of their conservation and aquaculture industry. Although DNA barcoding facilitates molecular identification and phylogenetic analysis of species, its availability in clariid catfish lineage remains uncertain. In this study, DNA barcoding was developed and validated for clariid catfish.

Long-Term Application of a Synbiotic Chitosan and KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish ( × ) during Winter.

The effects of potential synbiotic chitosan and KU011TH mixture on growth performance, immune response, and resistance were investigated for the first time. The control group was fed a basal diet (A), and group B was given the formula B diet that was supplemented with chitosan at 20 mL/kg diet via top dressing. The other synbiotic groups, C, D, and E, were top-dressed with the target probiotics at 1 × 10, 1 × 10, and 1 × 10 CFU/kg diet, respectively, and coated with the same concentration of chitosan.

Genomics-driven prophylactic measures to increase streptococcosis resistance in tilapia.

Streptococcosis caused by Streptococcus agalactiae and S. iniae is a significant problem that affects the success of tilapia aquaculture industries worldwide. In this critical review, we summarize the applicable practical strategies which may effectively enhance the world tilapia aquaculture development.

Mystifying Molecular Structure, Expression and Repertoire Diversity of IgM Heavy Chain Genes ( in Catfish and Hybrids: Two Novel Transcripts in Vertebrates.

Two novel immunoglobulin heavy chain () transcripts encoding membrane-bound forms of IgM (mIgM) were discovered in bighead catfish. The first transcript contains four constant and two transmembrane domains [Cμ1-Cμ2-Cμ3-Cμ4-TM1-TM2] that have never been reported in teleosts, and the second transcript is an unusual mIgM that has never been identified in any vertebrate [Cμ1-(Cδ2-Cδ3-Cδ4-Cδ5)-Cμ2-Cμ3-TM1-TM2]. Fluorescence hybridization (FISH) in bighead catfish, North African catfish () and hybrid catfish revealed a single copy of in individual parent catfish, while two gene copies were found in diploid hybrid catfish.

Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage.

The African catfish () may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex "switch" loci to determine sex within a species. Here, we aimed to detect the existence of PSD using hybrid.

A Current Update on the Distribution, Morphological Features, and Genetic Identity of the Southeast Asian Mahseers, Species.

The king of rivers or mahseer comprises three genera: , and under the Cyprinidae family. The genus has been classified as the true mahseer due to the presence of a median lobe among the three genera. The species are widely distributed across Southeast (SE) Asia, and 13 species have been reported previously: , , , and .

An Investigation of ZZ/ZW and XX/XY Sex Determination Systems in North African Catfish (, ).

An investigation of sex-specific loci may provide important insights into fish sex determination strategies. This may be useful for biotechnological purposes, for example, to produce all-male or all-female fish for commercial breeding. The North African catfish species, , has been widely adopted for aquaculture because its superior growth and disease resistance render the species suitable for hybridization with other catfish to improve the productivity and quality of fish meat.

Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM Cells in Catfish.

Catfish is a commonly-cultivated freshwater fish in Thailand and many Southeast Asian countries. The molecular data obtained for the IgM heavy chain (IgMH) of catfish have been useful for distinguishing monoclonal antibodies (mAbs). A mAb specific to Cμ of the IgMH of catfish (IgMHCμ mAb) was developed in a rabbit model using sequence information from bighead catfish ().

Transcriptomic analysis of female and male gonads in juvenile snakeskin gourami (Trichopodus pectoralis).

The snakeskin gourami (Trichopodus pectoralis) exhibits sexual dimorphism, particularly in body size. Since the snakeskin gourami is usually marketed during sexual maturation, the sexual size dimorphism has become an economically important trait. Sex-biased gene expression plays a key role in phenotypic sexual dimorphism.

Probiotic Effects of a Novel Strain, KU011TH, on the Growth Performance, Immune Responses, and Resistance against of Bighead Catfish ( Günther, 1864).

In the present study, the novel probiotic strain KU011TH with an evident lack of pathogenicity in catfish was experimented. Three practical administration routes, namely, feed additive (FD), water-soluble additive (SOL), and a combination route (FD+SOL), were applied in two sizes of catfish. After 120 days of FD+SOL administration, catfish fingerlings (15 g) exhibited a significant improvement in all tested growth performance parameters.

Strain KUO11TH, a Unique Organism Related to and Isolated from the Skin Mucus of Healthy Bighead Catfish and Its Efficacy Against Several Fish Pathogens.

The bacterial strain KU011TH was isolated from the skin mucus of healthy bighead catfish. The strain is a Gram-negative coccobacillus that is nonmotile, aerobic, catalase positive, oxidase negative, and nonhemolytic. Sequence analyses of the housekeeping genes , and indicate that this strain is a new member of the Acb complex of the genus and is closely related to and In addition the genome relatedness-associated ANIb (<95-96%) and DDH (<70%) values clearly supported the new member of the genus and the Acb complex.

Heritability of immunity traits and disease resistance of bighead catfish, Clarias macrocephalus Günther, 1864.

Disease outbreak is a major obstruction for intensive aquaculture worldwide. One of the promising solutions is genetic improvement by selective breeding, providing that a sufficient proportion of additive genetic variance (measured by heritability-h) of disease resistance traits exists. In addition, immunity traits are of interest as potential indirect targeted traits for disease resistance.

Pubertal effects of 17α-methyltestosterone on GH-IGF-related genes of the hypothalamic-pituitary-liver-gonadal axis and other biological parameters in male, female and sex-reversed Nile tilapia.

The influence of 17α-methyltestosterone (MT) on growth responses, biological parameters and the expression of genes involved in the GH-IGF pathway of the hypothalamic-pituitary-liver-gonadal axis were investigated in female, male, and sex-reversed Nile tilapia to evaluate the relationship between sex and MT-induced changes in these parameters. Female fish had a lower growth rate than male and sex-reversed fish, and MT increased growth performance and duodenal villi in females. Most but not all biological parameters of sex-reversed fish were similar to those of male fish.

DNA barcoding of catfish: species authentication and phylogenetic assessment.

As the global market for fisheries and aquaculture products expands, mislabeling of these products has become a growing concern in the food safety arena. Molecular species identification techniques hold the potential for rapid, accurate assessment of proper labeling. Here we developed and evaluated DNA barcodes for use in differentiating United States domestic and imported catfish species.

Spatial and temporal genetic variation of green mussel, Perna viridis in the Gulf of Thailand and implication for aquaculture.

The culture of green mussel (Perna viridis) in the Gulf of Thailand depends on natural spat which are believed to come from spawning grounds adjacent to major river mouths. In the present paper, genetic diversity of spatial and temporal populations of green mussel in the Gulf of Thailand was investigated using five microsatellite loci. The results showed moderate genetic variation of all 11 populations (averaged number of alleles per locus, A = 10.

Mitochondrial DNA diversity of the Asian moon scallop, Amusium pleuronectes (Pectinidae), in Thailand.

Sequence variation of the mitochondrial DNA 16S rRNA region of the Asian moon scallop, Amusium pleuronectes, was surveyed in seven populations along the coast of Thailand. A total of 16 unique haplotypes were detected among 174 individuals with a total 27 variable sites out of 534 bp sequenced. The mitochondrial haplotypes grouped into two distinct arrays (estimated to differ by about 2.

Study of genetic diversity of orange-spotted grouper, Epinephelus coioides, from Thailand and Indonesia using microsatellite markers.

Genetic diversity of Epinephelus coioides (Hamilton, 1822), which inhabits coastal reefs from the western Indian Ocean to the western Pacific Ocean, was studied based on four polymorphic microsatellite loci. Two hundred and fifty individuals were collected from two locations in Thailand (Nakornsrithammarat-N and Trang-T) and four in Indonesia (Sibolga-S, Lampung-L, Jepara-J, and Flores-F). The genetic variation of E.