Publications by authors named "Ute A Hoffmann"
RNA degradation is critical for synchronising gene expression with changing conditions in prokaryotic and eukaryotic organisms. In bacteria, the preference of the central ribonucleases RNase E, RNase J and RNase Y for 5'-monophosphorylated RNAs is considered important for RNA degradation. For RNase E, the underlying mechanism is termed 5' sensing, contrasting to the alternative 'direct entry' mode, which is independent of monophosphorylated 5' ends.
View Article and Find Full Text PDFSynthetic biology approaches toward the development of cyanobacterial producer strains require the availability of appropriate sets of plasmid vectors. A factor for the industrial usefulness of such strains is their robustness against pathogens, such as bacteriophages infecting cyanobacteria. Therefore, it is of great interest to understand the native plasmid replication systems and the CRISPR-Cas based defense mechanisms already present in cyanobacteria.
View Article and Find Full Text PDFArticle Synopsis
- - Ribonucleases are essential for RNA metabolism in bacteria, with cyanobacteria encoding key enzymes RNase E and RNase J; notably, cyanobacterial RNase E is shorter and lacks some domains found in other bacteria.
- - Researchers created a temperature-sensitive mutant of RNase E in the cyanobacterium Synechocystis sp. PCC 6803, allowing for investigation of its function through RNA sequencing after temporarily disabling it.
- - They discovered 1472 cleavage sites influenced by RNase E, revealing a specific RNA cleavage pattern and suggesting that RNase E works alongside RNase J to regulate mRNAs in cyanobacteria.