Effects of stroma-free haemoglobin solution on the blood-clotting system in dogs.

The effect of transfusions of 500 ml of 5-6% stroma-free haemoglobin solutions on the blood-clotting system of dogs was studied. Slight lowering of the fibrinogen level and of the activity of clotting factors V, VII, VIII, IX and X was observed, together with some prolongation of the thrombin time. In vitro investigations indicate that prolongation of the thrombin time may depend on disturbed polymerization of fibrin monomers.

The preparation of the factors IX, II, and X concentrate by means of DEAE cellulos and its therapeutic effectiveness.

Starting from a plasma used for producing cryoprecipitate a concentrate was gained by means of adsorption on DEAE cellulose, the factor IX activity of which was six to thirteen times higher than that of the original plasma. Even the factors X and II are enriched to the same extent approximately. In patients with haemophilia B the concentrate proved to be very efficient and compatible during surgical operations.