Study of the Efficiency of the Hydroporation for Delivery of Plasmid DNA to the Cells on the Model of Toxic Neuropathy.

We compared the efficiency of delivery of plasmid DNA (active ingredient concentration 1 mg/kg) that provides production of nerve growth factor (NGF) after intravenous administration to rats and after administration by hydroporation. The method of hydroporation ensured plasmid penetration into the liver tissue and lengthened the time of its detection in the organ. DNA concentration in 1 h after its introduction by hydroporation or intravenous route was 0.

Comparative Analysis of Polyethyleneimine Efficiency for Improvement of Plasmid DNA Bioavailability.

We studied the influence of the type and structure of polyethyleneimine on bioavailability and expression of plasmid DNA carrying IGF-1 gene. Polymers with different molecular weights (2.5, 10, 25, and 60 kDa) of linear and branching structure were studied.

Analysis of the Efficiency of Microencapsulated Sustained-Release Form of Naloxone on the Experimental Model of Fentanyl Poisoning.

We compared samples of microencapsulated naloxone prepared by using spray drying technique. 2-Hydroxypropyl-β-cyclodextrin, sodium alginate, polycaprolactone, and carboxymethyl cellulose were used as the carriers. It was found that the combination of naloxone with sodium alginate was characterized by the highest naloxone content in the matrix and the lowest release rate (100% release time was 60 min).

Effect of low-alcohol drinks on CNS structures in young animals.

Adaptation restructuring at the level of cells and cell populations in CNS in young animals after acute intoxication with a high dose of beer was evaluated by a complex of quantitative morphometric parameters. Variants of morphological manifestations of compensatory adaptive changes were studied and variability of their combinations in the neuroglial populations of the forebrain, cerebellum, and spinal cord in young animals was revealed reflecting plastic potentialities of the studied CNS structures exposed to a destructive factor.

[Thermal trauma in a combination with respiratory tract burn].

The detection rate of microbial contamination of burn wounds after flame burn was studied in dynamics to 3 weeks in 56 patients forming 2 similar groups: basic--with thermo-inhalation lesions (TIL) and control--without TIL. General area of skin burns in all the patients was less than 50% of the body surface, on average it was 40%, the area of deep burns was 23%. Cytological and bacteriologic methods were used for study microphlora in the sputum and in bronchoalveolar lavage.

[Experimental and clinical rationale for the use of atraumatic activated carbonic dressing in the treatment of burns].

Tests for functional properties of dressing material showed that in the degree of its atraumatic effect activated carbonated cloth takes an intermediate place between hydrophilic and hydrophobic materials. Atraumatic carbonated cloth is easily wettable with blood and drug solutions but absorbs them poorly. However, it exceeds medical gauze in the amount and fine mechanism of nitrafurazone adsorption whose molecules are chemically bound on the carbon fibre.

[Comparative radioautographic evaluation of RNA synthesis in vivo and in biopsy material].

The liver, kidney, skin biopsies are able to incorporate actively 3H-uridine, and the maximum rate of RNA synthesis over the hepatocyte nuclei in the peripheral zone of the biopsies corresponds to relevant rates in vivo. Only the skin biopsies were distinguished by total cell labelling. Liver, renal, skin capillary endotheliocytes can serve intertissue marker of the diffusion depth of 3H-uridine.

[Morphological characteristics of the healing of burn wounds covered by an activated-charcoal tissue dressing].

Activated-charcoal cloth and dressings product based upon this material were applied for covering burn wounds. As a control served a gauze dressings with greases and solution topical drugs. The healing of deep thermal burns of 3rd degree on rabbits studied by a histological method was differed by a rapid decreasing of inflammation reaction, active epithelialization and soft scar formation.

[Effects of gentamycin on interactions of neutrophils with Staphylococcus].

The authors studied the effect of gentamicin on abilities of neutrophils to uptake and killing of extracellular Staphylococcus. Gentamicin had not changed naturally the ability of healthy men and burn patients neutrophils to uptake Staphylococcus, but intensified the ability of neutrophils to killing of these bacteria.

[Electron-radioautographic study of phagocytosis of Pseudomonas aeruginosa by neutrophils].

RNA synthesis was studied in the bacteria and neutrophils from healthy persons having phagocytosis. Elevation of RNA synthesis occurred in the neutrophils with phagocytic activities. The rate of labelled RNA precursor incorporation into the bacteria was used as a measure of their viability.

[Radioautographic demonstration of the intracellular and extracellular bactericidal action of the neutrophils].

The authors described a method of quantitative radioautographic analysis of RNA synthesis level in the bacteria. The method allows to define by inhibition of RNA synthesis a bactericidal action of neutrophils during phagocytosis. The essence of a method consists in the introduction for a short period of a label and development of radioautographs at semithin section by paraphenylenediamine which form a small and numerous silver grains above bacteria which retained an ability to RNA synthesis.

[Effect of serum on the intra- and extracellular bactericidal capacity of neutrophils].

Donors' neutrophils are capable for inhibiting the growth of and killing staphylococci both intra- and extracellularly. In the absence of serum the rate of extracellular killing of microbes by donors' neutrophils is reaching that of killing phagocytized bacteria, while in the serum introduction the rate of intracellular killing overwhelms that of extracellular. Burnt patients' neutrophils fail to control the microbes multiplication either intra- or extracellularly without serum, while in combination with it both intra- and extracellular killing activity of neutrophils of the burnt sharply increases being superior to relevant combination of healthy subjects.

[Possibility of using activated carbon tissue in combination bandages].

The physico-chemical testing of charcoal cloth by means of adhesion and sorption techniques was performed. It was found that charcoal cloth with optimum activation degree can be used as dressing material. The morphological studies have shown an insignificant soft tissue response to activated charcoal implant in experimental animals and its in vivo stability at least for 2 months.