Publications by authors named "Usman Irshad"

is the central conifer plant affected by ozone and nitrogen pollutants among forest species worldwide. The growth of depends upon the ectomycorrhizal (ECM) association, which is usually disturbed by these factors. This study aims to understand how these factors affect plants at physiological and biochemical levels.

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The reduction in crop productivity due to drought stress, is a major concern in agriculture. Drought stress usually disrupts photosynthesis by triggering oxidative stress and generating reactive oxygen species (ROS). The use of zinc-quantum dot biochar (ZQDB) and proline (Pro) can be effective techniques to overcome this issue.

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Article Synopsis
  • Microalgae-based wastewater treatment systems show promise in reducing antibiotic resistance genes (ARGs) in municipal wastewater, with specific microalgae strains more effective than mixed cultures.
  • The study tracked 14 ARGs and found significant reductions in key genes within the first four days, highlighting correlations between specific ARGs and water quality parameters like total dissolved solids (TDS) and total suspended solids (TSS).
  • The research suggests that certain water quality indicators, particularly TDS and E. coli levels, can serve as cost-effective measures for monitoring ARG mitigation in wastewater treatment processes.
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Microalgal biomass provides a renewable source of biofuels and other green products. However, in order to realize economically viable microalgal biorefinery, strategic identification and utilization of suitable microalgal feedstock is fundamental. Here, a multi-step suboptimal screening strategy was used to target promising microalgae strains from selected freshwaters of the study area.

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Functioning of ecosystems depends on the nutrient dynamics across trophic levels, largely mediated by microbial interactions in the soil food web. The present study investigated the use of phosphate solubilizing bacteria (PSB) and poultry manure (PM) for maintaining labile P in the soil for an extensive fertility enhancement and as a substitution of chemical fertilizers. Based on the different P solubilizing capabilities of Bacillus and Pseudomonas, a quadruple consortium of Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis and Pseudomonas fluorescens, and their grazer nematodes (soil free living) supplemented with PM were studied.

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Article Synopsis
  • - The study investigates how irrigating food crops like tomatoes and chilies with industrial wastewater containing toxic metals leads to genetic changes and chromosomal abnormalities in the plants.
  • - Plants were exposed to varying metal concentrations (125-1000 mg/L), and their root tips were examined for chromosomal variations during mitosis, revealing issues such as laggards and binucleated cells.
  • - Results showed that higher metal exposure increased chromosomal aberrations and decreased the mitotic index, indicating that metal stress hampers cell division and causes genetic instability in the crops, with chilies showing more significant abnormalities than tomatoes.
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is economically and ethnobotanically an important forest tree and is shown to be at decline in Northern areas of Pakistan in recent years mainly due to high concentration of Nitrogen in forests. Ectomycorrhizal (ECM) association forming fungi enables the forest trees to develop optimally by absorbing water from the rhizosphere through their absorptive hyphae and by making available the nutrients by mobilization of N and P from the organic substrates. This study was conducted to identify the ECM strains from rhizosphere and to analyse the impact of high N load on the seedlings to establish N critical load value for coniferous forests of Pakistan.

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Low phosphorus soils are thought to constitute the majority of soils worldwide and cannot support intensive agriculture without high fertilizer inputs. Rhizobacteria are well-known to modify P dynamics and an increased bacterial diversity normally has a positive impact on various process rates. However, it is not known how variation in bacterial diversity at the subspecies level could influence trophic interactions in the rhizosphere and its consequences on plant P nutrition.

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Phosphorus (P) is essential for plant growth and productivity. It is one of the most limiting macronutrients in soil because it is mainly present as unavailable, bound P whereas plants can only use unbound, inorganic phosphate (Pi), which is found in very low concentrations in soil solution. Some ectomycorrhizal fungi are able to release organic compounds (organic anions or phosphatases) to mobilize unavailable P.

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