Generation of pulmonary arterial hypertension patient-specific induced pluripotent stem cell lines from three unrelated patients with a heterozygous missense mutation in exon 12, a heterozygous in-frame deletion in exon 3 and a missense mutation in exon 11 of the BMPR2 gene.

Loss-of-function mutations in the bone morphogenetic protein receptor 2 (BMPR2) gene are common in heritable or idiopathic pulmonary arterial hypertension (PAH), and can result in functional impairment of both endothelial and vascular smooth muscle cells. Here, we report 3 PAH patient-specific induced pluripotent stem cells (iPSC) lines from 3 unrelated patients harbouring different mutations in the BMPR2 gene: a heterozygous missense mutation in exon 12, a heterozygous frame shift deletion in exon 3, and a heterozygous missense mutation in exon 11. These cell lines will serve as a valuable resource to model PAH in vitro.

Sulfamethoxazole Leaching from Manure-Amended Sandy Loam Soil as Affected by the Application of Jujube Wood Waste-Derived Biochar.

Vertical translocation/leaching of sulfamethoxazole (SMZ) through manure-amended sandy loam soil and significance of biochar application on SMZ retention were investigated in this study. Soil was filled in columns and amended with manure spiked with 13.75 mg kg (S1), 27.

A National Survey to Assess the COVID-19 Vaccine-Related Conspiracy Beliefs, Acceptability, Preference, and Willingness to Pay among the General Population of Pakistan.

The current study aims to assess the beliefs of the general public in Pakistan towards conspiracy theories, acceptance, willingness to pay, and preference for the COVID-19 vaccine. A cross-sectional study was conducted through an online self-administered questionnaire during January 2021. The Chi-square test or Fisher exact test was utilized for statistical data analysis.

Biomarkers for Impending Risk of Osteoporosis in Premenopausal Women.

Objective: To compare 'cytokines' and 'bone turnover markers' in pre- and post-menopausal women and identify their relationship with bone mineral density (BMD) in both groups.  Study Design: A cross-sectional study.

Place And Duration Of Study: Department of Biological & Biomedical Sciences, The Aga Khan University, Karachi, Pakistan, from June 2017 to August 2019.

Synthesis, spectral analysis and biological evaluation of 2-{[(morpholin-4-yl)ethyl]thio}-5-phenyl/aryl-1,3,4-oxadiazole derivatives.

A series of new derivatives of 4-(2-chloroethyl)morpholine hydrochloride (5) were efficiently synthesized. Briefly, different aromatic organic acids (1a-f) were refluxed to acquire respective esters (2a-f) using conc. HSO as catalyst.

The excess insulin requirement in severe COVID-19 compared to non-COVID-19 viral pneumonitis is related to the severity of respiratory failure and pre-existing diabetes.

Introduction: Severe COVID-19 has been anecdotally associated with high insulin requirements. It has been proposed that this may be driven by a direct diabetogenic effect of the virus that is unique to SARS-CoV-2, but evidence to support this is limited. To explore this, we compared insulin requirements in patients with severe COVID-19 and non-COVID-19 viral pneumonitis.

First Experience Addressing the Prognostic Utility of Novel Urinary Biomarkers in Patients With COVID-19.

Urine 11-dehydro-thromboxane B (u11-dh-TxB), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and liver-type fatty acid binding protein levels (L-FABP) at the time of hospitalization were higher in coronavirus disease 2019 (COVID-19) patients with adverse events vs without events. Higher u11-dh-TxB and L-FABP levels were associated with longer hospitalization, more thrombotic events, and greater mortality, providing evidence for potential utility as early prognostic biomarkers for COVID-19.

Exploring the nanomechanical concepts of development through recent updates in magnetically guided system.

This article outlines an analytical analysis of unsteady mixed bioconvection buoyancy-driven nanofluid thermodynamics and gyrotactic microorganisms motion in the stagnation domain of the impulsively rotating sphere with convective boundary conditions. To make the equations physically realistic, zero mass transfer boundary conditions have been used. The Brownian motion and thermophoresis effects are incorporated in the nanofluid model.

Association between molecular subtype of local advanced breast cancer with Ca 15-3 level.

Background: Neoplasm is an abnormal mass of tissue that grows excessively and not coordinated with normal tissue growth and continues to do so even though the stimulation that triggered the change has stopped. Breast cancer can be known by using tumor marker, which has been used is mucin-like glycoprotein Carcinoma Antigen (CA 15-3) which is a tumor marker that is specific to breast cancer.

Method: This study is a cross-sectional study to determine the association between molecular subtypes of locally advanced breast cancer with CA 15-3 level at Abdul Wahab Sjahranie Samarinda Hospital.

Computational optimization for the deposition of bioconvection thin Oldroyd-B nanofluid with entropy generation.

The behavior of an Oldroyd-B nanoliquid film sprayed on a stretching cylinder is investigated. The system also contains gyrotactic microorganisms with heat and mass transfer flow. Similarity transformations are used to make the governing equations non-dimensional ordinary differential equations and subsequently are solved through an efficient and powerful analytic technique namely homotopy analysis method (HAM).

The effect of apis Dorsata honey as a complementary therapy to interleukin-6 (IL-6) levels and T lymphocytes of post-chemotherapy breast cancer patients.

Background: The overproduction of interleukin-6 (IL-6) in breast cancer cases can aggravate metastases. In comparison, the production of T lymphocytes plays a role in suppressing the development of tumor cells. Honey as a complementary therapy is expected to reduce the overproduction of IL-6 and facilitate the production of T lymphocytes in breast cancer cases.

The relationship of family support with decision-making duration in cancer patient to perform a mastectomy.

Background: Cancer is a disease caused by abnormal growth of body cells that turn malignant and continue to grow uncontrollably. One of the treatments for breast cancer is mastectomy. The quickness of decision-making determines the survival rate of prognosis patients.

Interaction of silver nanoparticles with catechol methyltransferase: Spectroscopic and simulation analyses.

Catechol O-methyltransferase, an enzyme involved in the metabolism of catechol containing compounds, catalyzes the transfer of a methyl group between S-adenosylmethionine and the hydroxyl groups of the catechol. Furthermore it is considered a potential drug target for Parkinson's disease as it metabolizes the drug levodopa. Consequently inhibitors of the enzyme would increase levels of levodopa.

The effect of human capital and physical capital on regional financial condition: the moderating effect of management control system.

This study examines the effect of Human Capital and Physical Capital on Regional Financial Condition with a Management Control System as moderator. Participants were employees of the Regional Financial and Asset Management Directorates located in three major cities in the province of South Sulawesi, Indonesia. The participants were recruited from three different regions resulting in 75 participants in a three-wave data collection procedure.

Production, Optimization, and Characterization of an Acid Protease from a Filamentous Fungus by Solid-State Fermentation.

Acid proteases represent an important group of enzymes, extensively used in food and beverage industries. There is an increased demand for acid proteases adapting to the industrial extreme environment, especially lower pH. Thus, this necessitates the search for a better acid protease from fungi that best performs in industrial conditions.

Development of intractable ascites due to thoracic duct hypertension.

We describe a 69-year-old dialysis-dependent patient who developed intractable ascites after zone 2 aortic reconstruction for a type IA thoracic endovascular aneurysm repair endoleak. Investigation as to the cause of ascites revealed a unique set of clinical circumstances leading to intractable bloody ascites. Investigation included imaging and invasive testing to diagnose the culprit mechanism.

Management of Adult Patients Supported with Venovenous Extracorporeal Membrane Oxygenation (VV ECMO): Guideline from the Extracorporeal Life Support Organization (ELSO).

Disclaimer: The use of venovenous extracorporeal membrane oxygenation (VV ECMO) in adults has rapidly increased worldwide. This ELSO guideline is intended to be a practical guide to patient selection, initiation, cannulation, management, and weaning of VV ECMO for adult respiratory failure. This is a consensus document which has been updated from the previous version to provide guidance to the clinician.

Mapping the role of pH-adjusted potassium in diabetic ketoacidosis: Hypokalemia and the patient outcomes.

Background: Incidence of hypokalemia during the management of diabetic ketoacidosis (DKA) is high despite detailed potassium replacement guidelines in its treatment.

Aim: We aimed to find the role of pH-adjusted potassium (pH ) in the development of hypokalemia, and their mutual impact on patient outcomes during DKA management.

Methodology: Adult DKA patient's admission data of preceding 3 years (2015-2017) were retrospectively clerked.

Comparative performance of extreme learning machine and Hammerstein-Weiner models for modelling the intestinal hyper-motility and secretory inhibitory effects of methanolic leaf extract of Diels (Combretaceae).

In this article, three data-driven approaches were explored, including two artificial intelligence (AI) based models namely; Extreme Learning Machine (ELM) and Hammerstein-Weiner (HW) models and a trivial linear model namely; multilinear regression (MLR). In this context, the models were developed using the onset of diarrhoea, the total number of wet faeces, total number of faeces, weight of intestinal content (g) and length of the small intestine (cm) as the independent variables. In contrast, distance travelled by charcoal meal (C) and volume of intestinal content (I) were considered as the dependent variables for the prediction of the intestinal hypermotility and secretory inhibitory effects of the methanol leaf extract of (MECH).

Changes in levels of cartilage oligomeric proteinase and urinary C-terminal telopeptide of type II collagen in subjects with knee osteoarthritis after dextrose prolotherapy: A randomized controlled trial.

Objective: To assess the effects of dextrose prolotherapy in patients with knee osteoarthritis on the levels of serum cartilage oligomeric proteinase and urinary C-terminal telopeptide of type II collagen, and on the Western Ontario McMaster Universities Index and numerical rating scale score for pain.

Methods: A randomized controlled trial, in which participants were randomly allocated into 2 groups, receiving injections of either hyaluronic acid or dextrose prolotherapy. The hyaluronic acid group received 5 injections, 1 each on weeks 1, 2, 3, 4 and 5, and the dextrose prolotherapy group received 3 injections, 1 each on weeks 1, 5 and 9.

Synergistic effect in concurrent removal of toxic methylene blue and acid red-1 dyes from aqueous solution by durian rind: kinetics, isotherm, thermodynamics, and mechanism.

In the present study, a synergistic effect between cationic methylene blue (MB) and anionic acid red 1 (AR1) on their concurrent adsorptive removal from aqueous binary solution onto durian rind (DR) was systematically investigated in batch mode across different parameters. The concurrent adsorption was pseudo-second-order kinetics and followed the Langmuir isotherm model, similar to their respective single component. The kinetics and intraparticle diffusion analyses demonstrated that the adsorption rate of MB was a 15-fold faster than AR1, and mass transports were governed by a combination of intraparticle and film diffusion.

Stabilization of heavy metals loaded sewage sludge: Reviewing conventional to state-of-the-art thermal treatments in achieving energy sustainability.

Sewage sludge has long been regarded as a hazardous waste by virtue of the loaded heavy metals and pathogens. Recently, more advanced technologies are introduced to make use of the nutrients from this hazardous sludge. Successful recovery of sludge's carbon content could significantly convert waste to energy and promote energy sustainability.