Standard terminology and labeling of ocular tissue for transplantation.

Purpose: To develop an internationally agreed terminology for describing ocular tissue grafts to improve the accuracy and reliability of information transfer, to enhance tissue traceability, and to facilitate the gathering of comparative global activity data, including denominator data for use in biovigilance analyses.

Methods: ICCBBA, the international standards organization for terminology, coding, and labeling of blood, cells, and tissues, approached the major Eye Bank Associations to form an expert advisory group. The group met by regular conference calls to develop a standard terminology, which was released for public consultation and amended accordingly.

An outbreak of acute post-cataract surgery Pseudomonas sp. endophthalmitis caused by contaminated hydrophilic intraocular lens solution.

Objective: To report the investigation for the source of infection and the clinical course and treatment response of 11 cases of acute post-cataract surgery endophthalmitis that developed during an outbreak.

Design: Retrospective, consecutive, interventional case series.

Participants: Eleven patients who developed acute postoperative endophthalmitis after an uneventful cataract surgery with intraocular lens implantation from September 6 to 29, 2010, at a tertiary eye care center in South India.

External ocular surface and lens microbiota in contact lens wearers with corneal infiltrates during extended wear of hydrogel lenses.

Objectives: To determine whether carriage of microbes on the contact lens or ocular surfaces during extended wear (EW) with soft hydroxyethyl methacrylate (HEMA)-based contact lenses predisposes the wearer to adverse events.

Methods: Participants (non-contact lens wearers) were enrolled in a clinical study involving wear of HEMA-based hydrogel lenses on a six night EW basis with weekly replacement. Type and number of bacteria colonizing the lower lid margins, upper bulbar conjunctiva, and contact lenses during EW after one night, 1 week, 1 month, and thereafter every 3 months for 3.

In vitro antibiotic susceptibility of rapidly growing nontuberculous mycobacteria isolated from patients with microbial keratitis.

Purpose: This study was undertaken to determine the antibiotic susceptibility and minimum inhibitory concentrations (MIC) of amikacin, tobramycin, ciprofloxacin, gatifloxacin, azithromycin, and clarithromycin against rapidly growing nontuberculous mycobacteria isolated from patients with keratitis.

Methods: A total of 15 rapidly growing nontuberculous mycobacteria isolated from corneal scrapings of keratitis patients from January 1999 through December 2007 were subjected to antimicrobial susceptibility testing by the E-Test to amikacin, tobramycin, ciprofloxacin, gatifloxacin, azithromycin, and clarithromycin.

Results: Out of 15 isolates, 13 were identified as Mycobacterium chelonae complex and 2 as Mycobacterium fortuitum complex.

Microbial keratitis after LASIK.

Purpose: To report cases of microbial keratitis after LASIK from a tropical climatic zone.

Methods: This retrospective case series included 17 eyes of 15 patients managed at our center from January 1999 to December 2007. All patients had a detailed clinical evaluation followed by corneal scrapings.

Clinical, microbiological profile and treatment outcome of ocular infections caused by Achromobacter xylosoxidans.

Purpose: To study the microbiological profile and outcome of ocular infections caused by Achromobacter xylosoxidans.

Methods: Medical and microbiology records of patients with A. xylosoxidans ocular infections managed between May 2007 and December 2007 were reviewed.

Review of epidemiological features, microbiological diagnosis and treatment outcome of microbial keratitis: experience of over a decade.

Purpose: To review the epidemiological characteristics, microbiological profile, and treatment outcome of patients with suspected microbial keratitis.

Materials And Methods: Retrospective analysis of a non-comparative series from the database was done. All the patients presenting with corneal stromal infiltrate underwent standard microbiologic evaluation of their corneal scrapings, and smear and culture-guided antimicrobial therapy.

Post-operative endophthalmitis due to an unusual pathogen, Comamonas testosteroni.

Here, we describe the first report of post-operative endophthalmitis due to Comamonas testosteroni in an elderly diabetic patient after complicated cataract surgery. The isolate was identified by using Mini API strips. The patient was successfully treated with intravitreal ceftazidime and oral ciprofloxacin.

Comparison of clinical and microbiological profiles in smear-positive and smear-negative cases of suspected microbial keratitis.

Purpose: To examine the hypothesis that initial smear examination results have a significant bearing on the management and outcome of suspected microbial keratitis.

Materials And Methods: One hundred and seventy consecutive patients with suspected microbial keratitis were included in a prospective nonrandomized comparative study and their detailed clinical and microbiological data (smears and cultures of corneal scrapings) were captured on a predesigned corneal ulcer database. Patients were divided into two groups: Group 1 included 68 patients with corneal scrapings negative in smears while Group 2 included 102 patients with corneal scrapings positive in smears.

Is microsporidial keratitis an emerging cause of stromal keratitis? A case series study.

Background: Microsporidial keratitis is a rare cause of stromal keratitis. We present a series of five cases of microsporidial keratitis from a single centre in southern India with microbiologic and histopathologic features.

Case Presentation: Patient charts of five cases of microsporidial stromal keratitis diagnosed between January 2002 and June 2004 were reviewed retrospectively for clinical data, microbiologic and histopathologic data.

Relationship between clinical presentation and visual outcome in postoperative and posttraumatic endophthalmitis in south central India.

Purpose: To determine risk factors for poor visual outcome in postoperative and posttraumatic endophthalmitis in a large referral center in south central India.

Methods: In this prospective observational series the authors examined 388 patients of postoperative (n= 206) and posttraumatic (n= 182) endophthalmitis at the L V Prasad Eye Institute in Hyderabad, India between 1991 and 1997. The analysis was confined to 236 patients-128 (62.

Ophthalmic applications of preserved human amniotic membrane: a review of current indications.

Preserved human amniotic membrane (AM) is currently being used for a wide spectrum of ocular surface disorders. The AM has a basement membrane, which promotes epithelial cell migration and adhesion. The presence of a unique avascular stromal matrix reduces inflammation, neovascularization and fibrosis.

Pigmented plaque presentation of dematiaceous fungal keratitis: a clinicopathologic correlation.

Purpose: To evaluate the clinical, microbiologic, and histopathologic features of dematiaceous fungal keratitis cases presenting with pigmented, raised, plaque-like infiltrate.

Study Design: Consecutive case series.

Methods: Microbiology-proven cases of fungal keratitis presenting with a pigmented infiltrate were included in the study.

Fungal infection of sutureless self-sealing incision for cataract surgery.

Purpose: To report the clinical picture and outcome of fungal infection of self-sealing wounds in cataract surgery.

Design: Retrospective noncomparative case series.

Participants: Seven postoperative cataract patients.

Clinical experience with N-butyl cyanoacrylate tissue adhesive in fungal keratitis.

Purpose: To evaluate the role of tissue adhesive in the management of corneal thinning or perforation associated with active fungal keratitis.

Design: Retrospective noncomparative interventional case series.

Methods: Seventy-three eyes of 73 patients with microbiologically proven keratomycosis associated with thinning or perforation participated in the study.

Rapid detection of fungal filaments in corneal scrapings by microwave heating-assisted Grocott's methenamine silver staining.

The Gomoris methanamine silver impregnation technique is a highly reliable and archiveable method of detecting fungal filaments, but the staining procedure is time consuming and laborious. A technique using microwave energy to reduce the duration of Gomori's silver staining is described.

Fungal keratitis associated with vernal keratoconjunctivitis.

Purpose: To report a case of fungal keratitis associated with vernal keratoconjunctivitis.

Case Report: A 22-year-old man with a history of vernal keratoconjunctivitis since October 1999 developed a shield ulcer in the left eye in June 2000, which resolved with intensive topical steroid therapy. He presented in August 2001 with onset of acute pain, redness, and decreased vision.

Anterior chamber tap: diagnostic and therapeutic indications in the management of ocular infections.

Purpose: To report three cases in which an anterior chamber tap was useful in the management of infection of the eye confined to the anterior segment.

Methods: In the first case, the patient presented with diffuse conjunctival congestion and thick anterior chamber exudates adhering to the back of the cornea. The second case involved fungal keratitis, and the patient was not responding to topical natamycin and systemic ketoconazole.

Evaluation of corneal scraping smear examination methods in the diagnosis of bacterial and fungal keratitis: a survey of eight years of laboratory experience.

Purpose: To determine the sensitivity, specificity, and predictive values of Gram and potassium hydroxide with calcofluor white (KOH+CFW) stains in the diagnosis of early and advanced microbial keratitis, a retrospective analysis of comparative data from a prospectively collected database was done.

Methods: Patients with nonviral microbial keratitis seen at L.V.

Evaluation of agent and host factors in progression of mycotic keratitis: A histologic and microbiologic study of 167 corneal buttons.

Purpose: To evaluate the host and agent factors in the progression of mycotic keratitis through the microbiologic evaluation and histologic study of human corneal buttons obtained at the time of therapeutic keratoplasty.

Design: Retrospective noncomparative consecutive case series.

Materials: One hundred sixty-seven corneal buttons from 148 patients of microbiologically diagnosed and treated cases of mycotic keratitis who underwent therapeutic keratoplasty between January 1995 and May 1998.

The epidemiological features and laboratory results of fungal keratitis: a 10-year review at a referral eye care center in South India.

Purpose: To report the epidemiological features and laboratory results of 1,352 cases of fungal keratitis diagnosed at the L.V. Prasad Eye Institute (LVPEI) in south India.