Publications by authors named "Urvi Thacker"
Precise regulation of DNA replication complex assembly requires cyclin-dependent kinase (CDK) and Dbf4-dependent kinase (DDK) activities to activate the replicative helicase complex and initiate DNA replication. Chemical probes have been essential in the molecular analysis of DDK-mediated regulation of MCM2-7 activation and the initiation phase of DNA replication. Here, the inhibitory activity of two distinct DDK inhibitor chemotypes, PHA-767491 and XL-413, were assessed in cell-free and cell-based proliferation assays.
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- - CIZ1 is a protein involved in critical nuclear functions like DNA replication and epigenetic maintenance, particularly associated with the inactive X-chromosome.
- - Researchers utilized molecular panning and mass spectrometry to identify CIZ1's protein interaction partners, highlighting significant connections with ribosomal subunits and nucleolar proteins, particularly DHX9.
- - The study reveals that CIZ1 and DHX9 interact and work together in the nucleolus, essential for cell cycle progression from G1 to S-phase, with their interaction heavily dependent on RNA polymerase I activity.
Article Synopsis
- The genome must be accurately copied for the health and longevity of multicellular organisms, and the cell cycle is regulated by cyclin-dependent kinases, with a focus on the role of Cip1 interacting Zinc finger protein 1 (Ciz1).
- Ciz1 plays a key role in the G1/S phase transition by interacting with components of the pre-replication complex, which helps to properly activate DNA replication and prevent unnecessary re-replication.
- Ciz1 is linked to cancer biology, as it is often overexpressed in tumors and essential for their growth, potentially contributing to cancer development by affecting cell cycle regulation and causing DNA replication stress, making it a potential target for cancer