Oligo-Not Only for Silencing: Overlooked Potential for Multidirectional Action in Plants.

Oligo technology is a low-cost and easy-to-implement method for direct manipulation of gene activity. The major advantage of this method is that gene expression can be changed without requiring stable transformation. Oligo technology is mainly used for animal cells.

Influence of Basalt Aggregate Crushing Technology on Its Geometrical Properties-Preliminary Studies.

The use of mineral aggregates is related to the increasing demand in construction, railway and road infrastructures. However, mineral aggregates can appear to be of variable quality, directly affecting their suitability for respective earthwork applications. Since the production of mineral aggregates should ensure the standardized, high-quality requirements of the final product, rock-crushing mechanisms should be investigated in a detailed manner.

Evaluation of the Structure and Geometric Properties of Crushed Igneous Rock Aggregates.

The aim of this publication is to analyze the influence of rock mineral composition and rock geometric properties on the quality of crushed aggregates, from the perspective of selecting an adequate aggregate production technology. This research is based on samples of crushed aggregates from plants processing igneous rocks from four different igneous deposits. In the case of the geometric properties, shape and flakiness indexes were identified and subsequently analyzed along with particle size distribution.

Wear and Fragmentation Resistance of Mineral Aggregates-A Review of Micro-Deval and Los Angeles Tests.

The aim of this article is to present the current knowledge and experiences related to wear and fragmentation resistance tests and to indicate those of their aspects that require further research. For this purpose, a review of the literature was performed. Results show that tests of resistance to wear (the M test) and fragmentation (the LA test) are performed worldwide according to different standards (and thus following different test methods), which prevents a comparison of the obtained results.

Small is beautiful: low activity alpha and gamma sources for small-scale radiation protection research experiments.

Purpose: Uncertainties regarding the magnitude of health effects following exposure to low doses of ionizing radiation remain a matter of concern both for professionals and for the public. There is consensus within the international radiation research community that more research is required on biological effects of radiation doses below 100 mGy applied at low dose rates. Moreover, there is a demand for increasing education and training of future radiation researchers and regulators.

Joint inhibition of mitochondrial complex IV and alternative oxidase by genetic or chemical means represses chloroplast transcription in .

Changes in the functional state of mitochondria have profound effects on other cellular compartments. Genome-wide expression analysis of mutants with an RNAi-silenced expression of mitoribosomal S10 protein has revealed extensive transcriptional reprogramming. A meta-analysis comparing expression datasets of 25 mitochondrial perturbations showed a high similarity of the mutant which is defective in the alternative oxidase (AOX1a) and dual-targeted mitochondrial and plastid RNA polymerase (RPOTmp), to .

Deficiency of mitoribosomal S10 protein affects translation and splicing in Arabidopsis mitochondria.

The ribosome is not only a protein-making machine, but also a regulatory element in protein synthesis. This view is supported by our earlier data showing that Arabidopsis mitoribosomes altered due to the silencing of the nuclear RPS10 gene encoding mitochondrial ribosomal protein S10 differentially translate mitochondrial transcripts compared with the wild-type. Here, we used ribosome profiling to determine the contribution of transcriptional and translational control in the regulation of protein synthesis in rps10 mitochondria compared with the wild-type ones.

[Ribosome profiling as an innovative tool to study the process of protein synthesis].

The ribosome profiling approach (Ribo-seq) is currently the most effective method to study the protein synthesis in vivo. This technique relies on sequencing of ribosome protected mRNA fragments (so-called ribosomal footprints) allowing to indicate the exact positions of ribosomes on transcripts. Advanced bioinformatic analysis of Ribo-seq data enables selection of ribosomal footprints originated from translating ribosomes, providing information about authentic translational status of mRNA.

Freeware tool for analysing numbers and sizes of cell colonies.

The clonogenic cell survival assay is a basic method to study the cytotoxic effect of radiation and chemical toxins. In large experimental setups, counting of colonies by eye is tiresome and prone to bias. Moreover, it is often interesting to quantify the size of individual colonies.

Biological effects of mixed-ion beams. Part 2: The relative biological effectiveness of CHO-K1 cells irradiated by mixed- and single-ion beams.

The relative biological effectiveness (RBE) values were determined for single- and mixed-ion beams containing carbon and oxygen ions. The CHO-K1 cells were irradiated with beams with the linear energy transfer (LET) values of 236-300 and 461-470 keV/μm for C and O ions, respectively. The RBE was estimated as a function of dose, survival fraction (SF) and LET.

Biological effects of mixed-ion beams. Part 1: Effect of irradiation of the CHO-K1 cells with a mixed-ion beam containing the carbon and oxygen ions.

Carbon and oxygen ions were accelerated simultaneously to estimate the effect of irradiation of living cells with the two different ions. This mixed ion beam was used to irradiate the CHO-K1 cells, and a survival test was performed. The type of the effect of the mixed ion beam on the cells was determined with the isobologram method, whereby survival curves for irradiations with individual ion beams were also used.

Investigation of the bystander effect in CHO-K1 cells.

Aim: Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1-4 Gy of high LET C ions and X-rays.

Background: The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology.

The influence of active exercise in low positions on the functional condition of the lumbar-sacral segment in patients with discopathy.

Background: Pain in the lower segments of the spine is among the most frequent symptoms in industrialized countries. Injuries to intervertebral discs are the cause of this kind of discomfort in 90% of cases. The factors promoting the disease are: physical activity limitation, prolonged sitting position, overweight and bad movement stereotypes.

Discopathy of the lumbar-sacral segment and its influence on sexual dysfunction.

Background: Intervertebral disc disease is a widespread medical and social problem. Degeneration of intervertebral discs can lead to disc disease, commonly known as discopathy. One of the consequences of discopathy is pressure on the spinal cord or nerve roots that supply the genitals and sexual centers located in the core.

The primary study on influence of fitness camps on rehabilitation efficiency of full laryngectomy patients.

The objective of this study was to estimate the efficiency of exercise driven rehabilitation procedure after laryngectomy. A retrospective review of case notes from the Ludwig Rydygier Hospital, Bydgoszcz, Poland archives was performed. The setting was the Rehabilitation Unit in Department of Occupational Therapy and Rehabilitation of Collegium Medicum, Nicolas Copernicus University, Poland.