Publications by authors named "Urszula Baginska"
Article Synopsis
- * The study introduces a high-throughput assay using a NPY-Nanoluc reporter to measure dense core vesicle (DCV) exocytosis, which aligns well with existing markers and operates effectively in both rodent and human neurons.
- * This new method shows improved sensitivity for detecting DCV exocytosis compared to traditional single-cell assays, making it valuable for drug screening and understanding mechanisms behind central nervous system disorders.
Article Synopsis
- Neuropeptides and neurotrophins, stored in dense core vesicles (DCVs), play a crucial role as chemical signals in the brain, but the factors affecting their fusion and replenishment are not fully understood.
- Research shows that stimulation patterns, particularly 50-Hz action potential bursts, lead to optimal DCV fusion, with shorter interburst intervals enhancing capacity without increasing total fusion.
- Different stimulation types, including theta burst and low-frequency stimulation, also affect DCV fusion, with more intense stimulation resulting in longer-lasting fusion events.
