Publications by authors named "Urja Joshi"
Falciparum malaria relies extensively on cell-to-cell communication, and earlier research on the function of exosomal proteins derived from infected red blood cells (iRBCs) has been classified into numerous important roles. In this study, the exosomes were derived from -iRBCs cultured in vitro during synchronized trophozoite stages. The isolated exosomes were assessed using NTA, FE-SEM, and flow cytometry.
View Article and Find Full Text PDFBackground: This study examines the feasibility and effects of introducing microRNA mimic into red blood cells (RBCs) at the initial phases of Plasmodium falciparum 3D7 (Pf3D7) infection. The aim is to determine the correlation between increased expression of miR-451a and parasitaemia.
Methods: In this study miR-mimic-451a labelled with Cy3 and transfected into control and infected RBCs using lipofectamine and analysed using the fluorescence microscopy and flow cytometry.
Article Synopsis
- - Erythrocytes, despite lacking nucleic acids, play significant roles in erythropoiesis and disease diagnosis, functioning for 120-140 days while metabolizing heme and managing oxidative stress.
- - These red blood cells are surprisingly rich in microRNAs (miRNAs), which can influence various health conditions like sickle cell anemia and cardiovascular diseases, though the exact mechanism of miRNA action in erythrocytes is still unclear.
- - The study of erythrocyte-derived exosomal miRNAs suggests they could be vital in understanding disease processes and developing non-invasive diagnostic markers, highlighting the need for further research in erythrocyte translational studies.
Plasmodium falciparum (P. falciparum), which causes the most severe form of malaria, if left untreated, has 24 h window in which it can cause severe illness and even death. The aim of this study was to create the most comprehensive and informative secretory-proteome possible by combining high-accuracy and high-sensitivity protein identification technology.
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- Immunization with radiation-attenuated Plasmodium sporozoites (RAS) can provide strong, long-lasting immunity against malaria, with intravenous (IV) injection being more effective than intradermal (ID) methods.
- In tests with mice, those immunized via IV were completely protected from malaria when challenged by either IV or ID, while ID-immunized mice showed lower protection levels and became susceptible under certain conditions.
- The study highlighted that the route of initial infection challenge impacts the durability of the immune response, with IV-immunized mice demonstrating a higher number of effective CD8 T cells, which are critical for maintaining immunity against malaria.
Immunization with radiation-attenuated sporozoites (RAS) shown to confer complete sterile protection against liver-stage (LS) infection that lasts about 6 to 9 months in mice. We have found that the intermittent infectious sporozoite challenge to immune mice following RAS vaccination extends the longevity of sterile protection by maintaining CD8 T cell memory responses to LS infection. It is reported that CD8α dendritic cells (DCs) are involved in the induction of LS-specific CD8 T cells following RAS or genetically attenuated parasite (GAP) vaccination.
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